Oudkhir M, Martelly I, Boilly B, Castagna M
Laboratoire de Biologie des Facteurs de Croissance, Université de Lille, France.
Biochem Biophys Res Commun. 1992 Apr 15;184(1):433-40. doi: 10.1016/0006-291x(92)91212-9.
Protein kinase C (PKC) activity was examined in the CNS of the newt Pleurodeles waltlii undergoing regeneration after limb amputation. In the spinal cord and brain of control newts, the level of PKC activity was virtually the same for the cytosolic and the particulate fractions. At days 7 and 14 after amputation of two limbs, a twofold increase in overall PKC activity occurred in the spinal cord and accounted for increased membrane-bound activity, while cytosolic activity was not significantly impaired. In contrast, overall PKC activity was not affected in brain. However, a twofold increase in the brain particulate fraction occurred at day 14 while cytosolic activity decreased proportionately. Similar alterations were observed in newts undergoing one or multiple limb amputations. Such changes in PKC activity neither occurred in the CNS of newt after limb denervation nor in the CNS of limb amputated frog Rana temporaria, an Amphibian which is unable to regenerate. Taken together, these results provide evidence that PKC of the CNS is involved in the regeneration process of newts. Changes in activation-associated PKC distribution proceeded through different mechanisms: long-lasting increase in membrane bound activity with a net increase of overall activity in the spinal cord, and long-term redistribution of enzyme activity to the particulate fraction in brain.
研究了肢体截肢后正在进行再生的蝾螈（Pleurodeles waltlii）中枢神经系统中的蛋白激酶C（PKC）活性。在对照蝾螈的脊髓和脑中，胞质部分和颗粒部分的PKC活性水平几乎相同。在两条肢体截肢后的第7天和第14天，脊髓中总的PKC活性增加了两倍，这是由于膜结合活性增加所致，而胞质活性没有明显受损。相比之下，脑中总的PKC活性没有受到影响。然而，在第14天，脑颗粒部分的活性增加了两倍，而胞质活性则相应降低。在进行单肢或多肢截肢的蝾螈中也观察到了类似的变化。这种PKC活性的变化在肢体去神经支配后的蝾螈中枢神经系统中未发生，在无法再生的两栖动物——肢体截肢的青蛙（Rana temporaria）的中枢神经系统中也未发生。综上所述，这些结果证明中枢神经系统的PKC参与了蝾螈的再生过程。与激活相关的PKC分布变化通过不同机制进行：脊髓中膜结合活性持续增加，总活性净增加；脑中酶活性长期重新分布到颗粒部分。
