反相液相色谱中的柱选择性。VIII. 苯基烷基柱和氟代柱。
Column selectivity in reversed-phase liquid chromatography. VIII. Phenylalkyl and fluoro-substituted columns.
作者信息
Marchand D H, Croes K, Dolan J W, Snyder L R, Henry R A, Kallury K M R, Waite S, Carr P W
机构信息
Department of Chemistry, University Wisconsin - River Falls, River Falls, WI 54022, USA.
出版信息
J Chromatogr A. 2005 Jan 7;1062(1):65-78. doi: 10.1016/j.chroma.2004.11.014.
As reported previously, five solute-column interactions (hydrophobicity, steric resistance, hydrogen-bond acidity and basicity, ionic interaction) quantitatively describe column selectivity for 163 alkyl-silica, polar-group and cyano columns. In the present study, solute retention and column selectivity for 11 phenyl and 5 fluoro-substituted columns were compared with alkyl-silica columns of similar ligand length. It is concluded that two additional solute-column interactions may be significant in affecting retention and selectivity for the latter columns: (a) dispersion interactions of varying strength as a result of significant differences in bonded-phase polarizability or refractive index and (b) pi-pi interactions in the case of phenyl columns and aromatic solutes. These 16 phenyl and fluoro columns were also characterized in terms of hydrophobicity, steric resistance, hydrogen-bond acidity and basicity, and ionic interaction.
如先前报道，五种溶质 - 固定相相互作用（疏水性、空间位阻、氢键酸度和碱度、离子相互作用）定量描述了163种烷基硅胶、极性基团和氰基柱的柱选择性。在本研究中，将11种苯基柱和5种氟取代柱的溶质保留和柱选择性与具有相似配体长度的烷基硅胶柱进行了比较。得出的结论是，另外两种溶质 - 固定相相互作用可能对影响后一种柱的保留和选择性具有重要意义：（a）由于键合相极化率或折射率存在显著差异而导致的不同强度的色散相互作用，以及（b）对于苯基柱和芳香族溶质而言的π-π相互作用。还根据疏水性、空间位阻、氢键酸度和碱度以及离子相互作用对这16种苯基柱和氟柱进行了表征。
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