Faggi E, Pini G, Campisi E
Department of Public Health, Microbiology Unit, University of Florence, Florence, Italy.
Mycoses. 2005 Jan;48(1):3-7. doi: 10.1111/j.1439-0507.2004.01030.x.
Authors compare two methods of extracting DNA from different fungi: the classic method with phenol/chloroform (P/C) and that with magnetic beads. Both were tested on Candida albicans and Cryptococcus neoformans var. neoformans, belonging to the yeast group and Microsporum canis, M. gypseum, Trichophyton rubrum, T. interdigitale, T. ajelloi, Epidermophyton floccosum, belonging to the dermatophytes group. Extraction products underwent polymerase chain reaction (PCR) fingerprinting with the appropriate primers to point out any disagreement in the genomic profiles. After having determined that the genomic profiles obtained from the DNA extracted from the same strain with the two methods correspond perfectly, the authors concluded that the extraction method with magnetic beads from fungal cells is simpler and quicker than with P/C extraction, greatly facilitating the obtainment of fungal DNA.
作者比较了两种从不同真菌中提取DNA的方法：经典的苯酚/氯仿（P/C）法和磁珠法。两种方法都在白色念珠菌、新型隐球菌新型变种（属于酵母组）以及犬小孢子菌、石膏样小孢子菌、红色毛癣菌、指间毛癣菌、阿耶洛毛癣菌、絮状表皮癣菌（属于皮肤癣菌组）上进行了测试。提取产物使用合适的引物进行聚合酶链反应（PCR）指纹分析，以指出基因组图谱中的任何差异。在确定用两种方法从同一菌株提取的DNA所获得的基因组图谱完全一致后，作者得出结论，从真菌细胞中提取DNA的磁珠法比P/C提取法更简单、更快，极大地便于获得真菌DNA。
