Irfan Muhammad, Yang Shuang, Yuxin Luo, Sun Jia-Xing
Biotechnology Research Laboratory, Shenyang Academy of Agricultural Sciences, Shenyang Liaoning, China.
Department of Biotechnology, University of Sargodha, Sargodha Pakistan.
Mol Biol Res Commun. 2017 Mar;6(1):27-31.
This study investigated the genetic diversity of morchella species using randomly amplified polymorphic DNA markers. Morchella species are an important group of edible mushrooms belonging to the family Helveliaceae with medicinal and economical significance. In this study we have developed an efficient method of genomic DNA isolation which was amplified by eight RAPD primers to test the polymorphism in three species of morchella. Out of all eight primers tested in current study, one of them (B8) resulted 100% polymorphism among the three studied species. Based on the RAPD profile a similarity matrix was generated to construct a dendrogram for phylogenetic analysis revealing the relationship among the three species of morchella.
本研究利用随机扩增多态性DNA标记研究了羊肚菌物种的遗传多样性。羊肚菌物种是马鞍菌科重要的可食用蘑菇类群，具有药用和经济价值。在本研究中，我们开发了一种高效的基因组DNA提取方法，并用8条RAPD引物进行扩增，以检测三种羊肚菌的多态性。在本研究测试的所有8条引物中，其中一条（B8）在三种研究物种中产生了100%的多态性。基于RAPD图谱生成相似性矩阵，构建系统发育分析的树状图，以揭示三种羊肚菌之间的关系。
