葡萄球菌肠毒素B（SEB）联合白细胞介素-1受体拮抗剂（IL-1ra）可延长高危角膜移植大鼠同种异体移植物的存活时间。

SEB combined with IL-1ra could prolong the survival of the rat allografts in high-risk corneal transplantation.

作者信息

Jie Y, Pan Z, Chen Y, Wei Y, Zhang W, Xu L, Wu Y, Peng H

机构信息

Beijing Institute of Ophthalmology and the Tongren Eye Bank, Beijing Tongren Eye Centre, Beijing Tongren Hospital, Capital University of Medical Science, Beijing, China.

出版信息

Transplant Proc. 2004 Dec;36(10):3267-71. doi: 10.1016/j.transproceed.2004.10.075.

DOI:10.1016/j.transproceed.2004.10.075

PMID:15686743

Abstract

PURPOSE

To determine whether the superantigen Staphylococcal enterotoxin B (SEB) combined with interleukin-1 receptor antagonist (IL-1ra) prolong allograft survival better than individual agents in high-risk corneal transplantation in a rat model.

METHODS

Fisher 344 donor corneas were transplanted into Lewis recipients. High-risk transplantation meant that the transplants were sutured into the recipient beds with corneal neovascularization induced by placing three interrupted sutures in the host cornea. All of the recipients were divided in blinded fashion into four groups. Group I was injected with saline buffer. Group II was injected intraperitoneally with 0.2 mL SEB (75 microg/kg) at 4-day intervals on three occasions before transplantation. Group III was injected with 0.1 mL IL-1ra (1 mg/mL) subconjunctivally from the first day after transplantation for 2 weeks. Group IV received both SEB and IL-1ra. All transplants were evaluated for signs of rejection for 4 weeks after surgery. Ten days after transplantation, two recipients in each group were sacrificed for histopathological and immunological evaluation.

RESULTS

The mean survival time of the allografts in the control group was 5.89 +/- 0.79 days; in SEB group, 10.70 +/- 2.52 days; in IL-1ra group, 8.25 +/- 0.71 days; in the SEB and IL-1ra group, 17.36 +/- 2.39 days. CD4+ and CD8+ lymphocyte infiltration into the allografts and the percentage of the lymphocytes in the spleen and mandibular lymphatic nodes was significantly decreased among the treated groups with dampened lymphocyte reactivity. The SEB plus IL-1ra combination group showed the strongest inhibition.

CONCLUSION

SEB and IL-1ra are most effective in combination to treat high-risk corneal transplants.

摘要

目的

在大鼠高危角膜移植模型中，确定超抗原葡萄球菌肠毒素B（SEB）与白细胞介素-1受体拮抗剂（IL-1ra）联合使用是否比单独使用药物能更好地延长同种异体移植物的存活时间。

方法

将Fisher 344供体角膜移植到Lewis受体中。高危移植是指通过在宿主角膜中放置三根间断缝线诱导角膜新生血管形成后，将移植物缝合到受体床中。所有受体以盲法分为四组。第一组注射生理盐水缓冲液。第二组在移植前每隔4天腹腔注射0.2 mL SEB（75 μg/kg），共注射三次。第三组从移植后第一天开始结膜下注射0.1 mL IL-1ra（1 mg/mL），持续2周。第四组同时接受SEB和IL-1ra。术后4周对所有移植物进行排斥反应迹象评估。移植后10天，每组处死两只受体进行组织病理学和免疫学评估。

结果

对照组同种异体移植物的平均存活时间为5.89±0.79天；SEB组为10.70±2.52天；IL-1ra组为8.25±0.71天；SEB和IL-1ra组为17.36±2.39天。在淋巴细胞反应性受到抑制的治疗组中，同种异体移植物中CD4 +和CD8 +淋巴细胞浸润以及脾脏和下颌淋巴结中淋巴细胞的百分比显著降低。SEB加IL-1ra联合组显示出最强的抑制作用。