Jie Y, Pan Z, Chen Y, Wei Y, Zhang W, Wu Y, Peng H, Xu L
Beijing Institute of Ophthalmology, TongRen Eye Center, TongRen Hospital, Capital University of Medical Sciences, China.
Br J Ophthalmol. 2005 Mar;89(3):364-8. doi: 10.1136/bjo.2004.048959.
To explore the role of staphylococcal enterotoxin B (SEB) in treating high risk corneal keratoplasty in rats.
Rat corneal high risk transplantation rejection models were set up using Fisher 344 and Lewis rats. The experimental rats were injected intraperitoneally with 0.2 ml SEB at different concentrations before keratoplasty. The rejection indexes of the allograft were recorded and the lymphocyte infiltration in the allograft and the percentage of the lymphocyte subpopulation in the lymphatic organs were also examined. Lymphocyte proliferation ability and the concentration of IL-2 and IL-10 in the serum were also evaluated.
Compared with the control group, SEB prolonged the survival time of the allograft significantly from 7 to 12 days. It could also reduce CD4(+) and CD8(+) lymphocyte infiltration in the allograft and minimise the percentage of CD4(+) and CD8(+) lymphocytes in the lymphatic organs. The lymphocyte proliferation ability was also weakened. However, the percentage of CD4(+) NK T lymphocytes in the lymphatic organs was raised. The serum concentration of IL-10 was higher but IL-2 was lower in the SEB treated groups.
SEB prolonged the survival time of the allograft in high risk rat corneal allo-transplantation, which may be caused by T cell deletion and acquisition of non-specific tolerance.
探讨葡萄球菌肠毒素B（SEB）在大鼠高危角膜移植术中的作用。
采用Fisher 344和Lewis大鼠建立大鼠角膜高危移植排斥模型。在角膜移植术前，给实验大鼠腹腔注射不同浓度的0.2 ml SEB。记录同种异体移植物的排斥指数，并检测同种异体移植物中的淋巴细胞浸润以及淋巴器官中淋巴细胞亚群的百分比。还评估了淋巴细胞增殖能力以及血清中IL-2和IL-10的浓度。
与对照组相比，SEB显著延长了同种异体移植物的存活时间，从7天延长至12天。它还可以减少同种异体移植物中CD4(+)和CD8(+)淋巴细胞浸润，并使淋巴器官中CD4(+)和CD8(+)淋巴细胞的百分比降至最低。淋巴细胞增殖能力也减弱。然而，淋巴器官中CD4(+) NK T淋巴细胞的百分比升高。SEB治疗组血清中IL-10浓度较高而IL-2浓度较低。
SEB延长了高危大鼠角膜同种异体移植中同种异体移植物的存活时间，这可能是由T细胞缺失和获得非特异性耐受性引起的。
