利用细菌人工染色体阵列比较基因组杂交技术绘制实验室小鼠的节段性和序列变异图谱。
Mapping segmental and sequence variations among laboratory mice using BAC array CGH.
作者信息
Snijders Antoine M, Nowak Norma J, Huey Bing, Fridlyand Jane, Law Sindy, Conroy Jeffrey, Tokuyasu Taku, Demir Kubilay, Chiu Readman, Mao Jian-Hua, Jain Ajay N, Jones Steven J M, Balmain Allan, Pinkel Daniel, Albertson Donna G
机构信息
Cancer Research Institute, University of California San Francisco, San Francisco, California 94143, USA.
出版信息
Genome Res. 2005 Feb;15(2):302-11. doi: 10.1101/gr.2902505.
We used arrays of 2069 BACs (1303 nonredundant autosomal clones) to map sequence variation among Mus spretus (SPRET/Ei and SPRET/Glasgow) and Mus musculus (C3H/HeJ, BALB/cJ, 129/J, DBA/2J, NIH, FVB/N, and C57BL/6) strains. We identified 80 clones representing 74 autosomal loci of copy number variation (|log(2)ratio| >/= 0.4). These variant loci distinguish laboratory strains. By FISH mapping, we determined that 63 BACs mapped to a single site on C57BL/6J chromosomes, while 17 clones mapped to multiple chromosomes (n = 16) or multiple sites on one chromosome (n = 1). We also show that small ratio changes (Delta log(2)ratio approximately 0.1) distinguish homozygous and heterozygous regions of the genome in interspecific backcross mice, providing an efficient method for genotyping progeny of backcrosses.
我们使用了由2069个细菌人工染色体（BAC）（1303个非冗余常染色体克隆）组成的阵列，来绘制家鼠（SPRET/Ei和SPRET/格拉斯哥）和小家鼠（C3H/HeJ、BALB/cJ、129/J、DBA/2J、NIH、FVB/N和C57BL/6）品系之间的序列变异图谱。我们鉴定出了80个克隆，它们代表了74个拷贝数变异的常染色体基因座（|log(2)比值|≥0.4）。这些变异基因座区分了实验室品系。通过荧光原位杂交（FISH）定位，我们确定63个BAC定位于C57BL/6J染色体上的单个位点，而17个克隆定位于多条染色体（n = 16）或一条染色体上的多个位点（n = 1）。我们还表明，小的比值变化（Δlog(2)比值约为0.1）可区分种间回交小鼠基因组中的纯合和杂合区域，为回交后代的基因分型提供了一种有效的方法。
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