Dejmek Annika, Hjerpe Anders
Department of Pathology and Cytology, Malmö University Hospital, Lund University, Sweden.
Diagn Cytopathol. 2005 Mar;32(3):160-6. doi: 10.1002/dc.20202.
A previously tested antibody panel identified three criteria of major importance for distinguishing between mesothelioma and adenocarcinoma (ACA): carcinoembryonic antigen (CEA), BerEp4, and epithelial membrane antigen (EMA) accentuated at the cell membrane. An extended panel, consisting of CEA, BerEp4, EMA, vimentin, mesothelioma antibody (HBME-1), thrombomodulin, Ca125, and sialyl-Tn was applied to effusions from 86 ACAs and 21 mesotheliomas. The specificities and sensitivities of the previously identified reactivity patterns were tested on the new material and the effect of the added antibodies was evaluated. Further, hyaluronan analysis was added as a parameter. The previously selected criteria remained fully predictive for mesothelioma and ACA, respectively, also in the extended material (in all, 139 ACAs and 57 mesotheliomas). With the addition of the hyaluronan value, 79% of the cases was identified with 100% specificity. Among the new antibodies sialyl-Tn seemed the most promising because it specifically identified ACAs not expressing CEA.
一个先前经过测试的抗体组合确定了区分间皮瘤和腺癌（ACA）的三个至关重要的标准：癌胚抗原（CEA）、BerEp4以及细胞膜上显著表达的上皮膜抗原（EMA）。一个扩展的抗体组合，包括CEA、BerEp4、EMA、波形蛋白、间皮瘤抗体（HBME-1）、血栓调节蛋白、Ca125和唾液酸-Tn，被应用于86例腺癌和21例间皮瘤的积液样本。在新的样本材料上测试了先前确定的反应模式的特异性和敏感性，并评估了新增抗体的作用。此外，添加了透明质酸分析作为一个参数。在扩展后的样本材料中（总共139例腺癌和57例间皮瘤），先前选定的标准分别对间皮瘤和腺癌仍具有完全的预测性。加上透明质酸值后，79%的病例被确诊，特异性为100%。在新增的抗体中，唾液酸-Tn似乎最具前景，因为它能特异性识别不表达CEA的腺癌。
