Miyoshi Tatsuo, Iwatsuki Teruki, Naganuma Takeshi
Graduate School of Biosphere Science, Hiroshima University, 1-4-4 Kagamiyama, Higashi-Hiroshima 739-8528, Japan.
Appl Environ Microbiol. 2005 Feb;71(2):1084-8. doi: 10.1128/AEM.71.2.1084-1088.2005.
A total of 247 clones of 16S rRNA genes from microorganisms captured by 0.2- and 0.1-microm-pore-size filters from sedimentary and granite rock aquifers were amplified and yielded 37 operational taxonomic units (OTUs). Fifteen OTUs captured by 0.1-microm-pore-size filters were affiliated with the candidate divisions OD1 and OP11, representing novel lineages. On the other hand, OTUs captured by 0.2-microm-pore-size filters were largely affiliated with Betaproteobacteria.
从沉积岩和花岗岩含水层中通过0.2微米和0.1微米孔径过滤器捕获的微生物的16S rRNA基因共247个克隆被扩增，产生了37个操作分类单元（OTU）。0.1微米孔径过滤器捕获的15个OTU与候选分类群OD1和OP11相关，代表新的谱系。另一方面，0.2微米孔径过滤器捕获的OTU主要与β-变形菌纲相关。
