Hugenholtz Philip, Huber Thomas
ComBinE group, Advanced Computational Modelling Centre, The University of Queensland, Brisbane 4072, Australia.
Int J Syst Evol Microbiol. 2003 Jan;53(Pt 1):289-293. doi: 10.1099/ijs.0.02441-0.
A significant number of chimeric 16S rDNA sequences of diverse origin were identified in the public databases by partial treeing analysis. This suggests that chimeric sequences, representing phylogenetically novel non-existent organisms, are routinely being overlooked in molecular phylogenetic surveys despite a general awareness of PCR-generated artefacts amongst researchers.
通过部分系统发育分析，在公共数据库中鉴定出了大量来源各异的嵌合16S rDNA序列。这表明，尽管研究人员普遍意识到PCR产生的假象，但代表系统发育上新的不存在生物的嵌合序列在分子系统发育调查中经常被忽视。
