Liebana Ernesto, Smith Richard P, Batchelor Miranda, McLaren Ian, Cassar Claire, Clifton-Hadley Felicity A, Paiba Giles A
Department of Food and Environment Safety, Veterinary Laboratories Agency--Weybridge, Addlestone, Surrey UK.
J Clin Microbiol. 2005 Feb;43(2):898-902. doi: 10.1128/JCM.43.2.898-902.2005.
We performed pulsed-field gel electrophoresis on Escherichia coli O157 isolates (n = 318) from 199 healthy animals in a longitudinal study carried out on nine farms. Investigation of the restriction types proved that at the farm level, the same clones can be detected on sampling occasions separated by as much as 17 months. The cohort animals were repeatedly sampled, and for some of these, the same clones were obtained on sampling occasions separated by as much as 8 months.
在对九个农场进行的一项纵向研究中,我们对来自199只健康动物的大肠杆菌O157分离株(n = 318)进行了脉冲场凝胶电泳。对限制性类型的调查证明,在农场层面,在相隔长达17个月的采样时间可以检测到相同的克隆。对同群动物进行了多次采样,其中一些动物在相隔长达8个月的采样时间获得了相同的克隆。