Boehm T L, Drahovsky D
Z Naturforsch C Biosci. 1979 May-Jun;34C(5-6):436-41. doi: 10.1515/znc-1979-5-620.
The sequence complexity of nuclear RNA from mouse liver, mouse spleen and highly malignant P815 mastocytoma was measured by nRNA driven hybridization to unique DNA sequences of P815 cells. The unique DNA sequences represent 63% of the total nuclear DNA of P815 cells and their availibility in hybridization experiments was found to be 76%. Of these sequences 7.8% formed hybrids with nuclear RNA of this cell, about 11.5% with mouse spleen and about 14.5% with mouse liver nuclear RNA. Assuming an asymmetrical transcription, the complexities of these transcripts are 2.8 X 10(8) nucleotides for mouse P815 mastocytomas, 4.3 X 10(8) for mouse spleen and about 5.3 X 10(8) nucleotides for mouse liver. Cellular specifity of the transcribed information was analyzed in additivity experiments, in which unique DNA sequences, not complementary to the nuclear RNA of one cell were annealed to the nuclear RNAs of the two other tissues/cells. In these experiments most of the nuclear RNA sequences of P815 cells were found to be also present in the nucleus of mouse liver and spleen. Only a small portion of the unique DNA sequences of P815 mastocytoma (about 1.2% corresponding to 4.4 X 10(7) nucleotides) was found to be complementary only to P815 mastocytoma nuclear RNA.
通过用P815细胞的单一DNA序列与小鼠肝脏、小鼠脾脏及高恶性P815肥大细胞瘤的核RNA进行杂交，测定了它们的序列复杂性。这些单一DNA序列占P815细胞总核DNA的63%，并且发现在杂交实验中其可用性为76%。在这些序列中，7.8%与该细胞的核RNA形成杂交体，约11.5%与小鼠脾脏的核RNA形成杂交体，约14.5%与小鼠肝脏的核RNA形成杂交体。假设转录是不对称的，对于小鼠P815肥大细胞瘤，这些转录本的复杂性为2.8×10⁸个核苷酸，小鼠脾脏为4.3×10⁸个核苷酸，小鼠肝脏约为5.3×10⁸个核苷酸。在加性实验中分析了转录信息的细胞特异性，在该实验中，将与一种细胞的核RNA不互补的单一DNA序列与另外两种组织/细胞的核RNA退火。在这些实验中，发现P815细胞的大多数核RNA序列也存在于小鼠肝脏和脾脏的细胞核中。仅发现P815肥大细胞瘤的一小部分单一DNA序列（约1.2%，相当于4.4×10⁷个核苷酸）仅与P815肥大细胞瘤的核RNA互补。
