Kleiman L, Birnie G D, Young B D, Paul J
Biochemistry. 1977 Mar 22;16(6):1218-23. doi: 10.1021/bi00625a029.
The base-sequence complexities of polysomal poly(A)+ RNA, nuclear poly(A)+ RNA, and total nuclear RNA from Friend erythroleukemia cells in logarithmic phase of growth were determined by measuring the proportion of labeled unique mouse DNA sequences which formed hybrids when incubated with a vast excess of each RNA. It was estimated that the RNAs had been transcribed from 1.8, 7.6, and 8.3%, respectively, of the haploid mouse genome. Although these estimates for polysomal and nuclear poly(A)+ RNAs were 2.5 times greater than those previously determined by analysis of the kinetics of the hybridization reactions between the RNAs and the cDNAs transcribed from them, they confirmed that the base-sequence complexity of nuclear poly(A)+ RNA in these cells is at least four times greater than that of polysomal poly(A)+ RNA.
通过测量与大量过量的每种RNA孵育时形成杂交体的标记独特小鼠DNA序列的比例，确定了处于对数生长期的Friend红白血病细胞的多聚核糖体多聚腺苷酸（poly(A)+）RNA、核多聚腺苷酸（poly(A)+）RNA和总核RNA的碱基序列复杂度。据估计，这些RNA分别来自单倍体小鼠基因组的1.8%、7.6%和8.3%。尽管这些针对多聚核糖体和核多聚腺苷酸（poly(A)+）RNA的估计值比之前通过分析RNA与从它们转录而来的cDNA之间杂交反应的动力学所确定的值大2.5倍，但它们证实了这些细胞中核多聚腺苷酸（poly(A)+）RNA的碱基序列复杂度至少是多聚核糖体多聚腺苷酸（poly(A)+）RNA的四倍。
