Tishkov V I, Khoronenkova S V
Department of Chemical Enzymology, Faculty of Chemistry, Lomonosov Moscow State University, Moscow 119992, Russia.
Biochemistry (Mosc). 2005 Jan;70(1):40-54.
D-Amino acid oxidase (DAAO) is a FAD-dependent enzyme that plays an important role in microbial metabolism, utilization of endogenous D-amino acids, regulation of the nervous system, and aging in mammals. DAAO from yeasts Rhodotorula gracilis and Trigonopsis variabilis are used to convert cephalosporin C into 7-aminocephalosporanic acid, the precursor of other semi-synthetic cephalosporins. This review summarizes the recent data on the enzyme localization, physiological role, gene cloning and expression, and the studies on the enzyme structure, stability, catalytic mechanism, and practical applications.
D-氨基酸氧化酶（DAAO）是一种依赖黄素腺嘌呤二核苷酸（FAD）的酶，在微生物代谢、内源性D-氨基酸的利用、神经系统调节以及哺乳动物衰老过程中发挥着重要作用。来自纤细红酵母和可变三角酵母的DAAO被用于将头孢菌素C转化为7-氨基头孢烷酸，后者是其他半合成头孢菌素的前体。本文综述了关于该酶的定位、生理作用、基因克隆与表达，以及酶结构、稳定性、催化机制和实际应用等方面的最新数据。
