转录因子与DNA相互作用的基因组学研究。

Genomic studies of transcription factor-DNA interactions.

作者信息

Sikder Devanjan, Kodadek Thomas

机构信息

Department of Internal Medicine, Center for Biomedical Inventions, University of Texas Southwestern Medical Center, 5323 Harry Hines Blvd, Dallas, Texas 75390-8573, USA.

出版信息

Curr Opin Chem Biol. 2005 Feb;9(1):38-45. doi: 10.1016/j.cbpa.2004.12.008.

DOI:10.1016/j.cbpa.2004.12.008

PMID:15701451

Abstract

A central issue in the regulation of gene expression is the physical association of transcription factors with relevant promoter sequences. Recently, technological advances have allowed researchers to analyze these processes on a genomic scale. In particular, the combination of the chromatin immunoprecipitation (ChIP) technique with microarray analysis (the 'ChIP to chip' experiment) is providing a wealth of new and surprising data on transcription factor-chromatin interactions. These advances are reviewed here. We also discuss future challenges in the area.

摘要

基因表达调控中的一个核心问题是转录因子与相关启动子序列的物理关联。最近，技术进步使研究人员能够在基因组规模上分析这些过程。特别是，染色质免疫沉淀（ChIP）技术与微阵列分析（“ChIP芯片”实验）的结合，正在提供大量关于转录因子与染色质相互作用的新的、令人惊讶的数据。本文对这些进展进行了综述。我们还讨论了该领域未来面临的挑战。