Udden M M
Hematology/Oncology Section, Baylor College of Medicine, One Baylor Plaza, Houston, TX 77030, USA.
Toxicol Lett. 2005 Mar 28;156(1):95-101. doi: 10.1016/j.toxlet.2003.09.021.
The toxicity of diethylene glycol butyl ether (DGBE), and its principal metabolite, butoxyethoxyacetic acid (BEAA), were assessed in vitro for rat and human red blood cells. Rat erythrocytes showed evidence of mild hemolysis when exposed to BEAA at concentrations of 5 or 10 mM for 4 h. BEAA treated rat red blood cells also showed evidence of sub-hemolytic damage: increased spherocytosis, a shift in distribution of cell size to larger cells, a significant increase in mean cellular volume, and a decrease in cellular deformability. However, DGBE had no effect on rat red blood cell morphology, cell size, hemolysis or deformability. There was no hemolysis when human red blood cells were exposed to DGBE or BEAA at the same concentrations. No changes in mean cellular volume, distribution of cell size, or morphologic appearance of human red blood cells were observed. No evidence for decreased deformability of human red blood cells exposed to DGBE or BEAA was found. In conclusion, BEAA has weak hemolytic activity and sub-hemolytic effects in vitro on rat erythrocytes, which is consistent with the finding of mild hemolysis when the parent compound DGBE is administered to rats by gavage. The absence of hemolysis or sub-hemolytic damage when human red blood cells were exposed to BEAA or DGBE in vitro indicates that it is unlikely that hemolysis will occur as a result of human exposure to DGBE.
在体外对大鼠和人类红细胞评估了二乙二醇丁醚（DGBE）及其主要代谢产物丁氧基乙氧基乙酸（BEAA）的毒性。当大鼠红细胞在5或10 mM浓度下暴露于BEAA 4小时时，出现了轻度溶血的迹象。经BEAA处理的大鼠红细胞还显示出亚溶血损伤的迹象：球形红细胞增多、细胞大小分布向较大细胞转移、平均细胞体积显著增加以及细胞变形性降低。然而，DGBE对大鼠红细胞形态、细胞大小、溶血或变形性没有影响。当人类红细胞暴露于相同浓度的DGBE或BEAA时，未发生溶血。未观察到人类红细胞的平均细胞体积、细胞大小分布或形态外观发生变化。未发现暴露于DGBE或BEAA的人类红细胞变形性降低的证据。总之，BEAA在体外对大鼠红细胞具有较弱的溶血活性和亚溶血作用，这与经口灌胃给予母体化合物DGBE时大鼠出现轻度溶血的结果一致。当人类红细胞在体外暴露于BEAA或DGBE时未发生溶血或亚溶血损伤，这表明人类接触DGBE不太可能导致溶血。
