Zhang Zi-xue, Ding Wei-qun, Tang Yong, Shi Wei-jing, Ye Wen-cai
Anhui Technical Teacher College, Fengyang 233100 Anhui, China.
Zhongguo Zhong Yao Za Zhi. 2004 Mar;29(3):215-8.
To speed up seedling production of pasqueflower (Puzlsatilla chinenses) and their modernization in pasqueflower.
With tissue culture method, primary culture of different explants, culture of cluster buds and their rooting culture were conducted on medium of treatment combinations of adding different hormones.
The appropriate medium for different culture stages were MS + 6-BA 1.0-3.0 mg x L(-1) + NAA 0-0.05 mg x L(-1) + Sucrose 30 g x L(-1) in primary culture, MS + 6-BA 0.2 mg x L(-1) + NAA 0.02 mg x L(-1) + BR 0.00001 mg x L(-1) + Sucrose 30 g x L(-1) in differentiation and subculture of cluster buds, 1/2 MS + NAA 0.4 mg x L(-1) + Sucrose 20 g x L(-1) in rooting.
Applying stem tip and flower buds as explants, high frequency propagation of seedlings can be achieved with plant tissue culture in Pasqueflower.
加快白头翁幼苗生产及其现代化进程。
采用组织培养方法,在添加不同激素的处理组合培养基上进行不同外植体的初代培养、丛生芽培养及其生根培养。
初代培养适宜培养基为MS + 6 - BA 1.0 - 3.0 mg·L⁻¹ + NAA 0 - 0.05 mg·L⁻¹ + 蔗糖30 g·L⁻¹;丛生芽分化与继代培养适宜培养基为MS + 6 - BA 0.2 mg·L⁻¹ + NAA 0.02 mg·L⁻¹ + BR 0.00001 mg·L⁻¹ + 蔗糖30 g·L⁻¹;生根适宜培养基为1/2 MS + NAA 0.4 mg·L⁻¹ + 蔗糖20 g·L⁻¹。
以茎尖和花蕾为外植体,通过植物组织培养可实现白头翁幼苗的高频繁殖。