[真菌诱导子对人参毛状根生物合成人参皂苷及生物量影响的研究]
[Studies on influence of fungal elicitor on hairy root of Panax ginseng biosynthesis ginseng saponin and biomass].
作者信息
Liu Jun, Ding Jia-yi, Zhou Qian-yun, He Lin, Wang Zheng-tao
机构信息
School of Traditional Chinese Medicine, China Pharmaceutical University, Nanjing 210038, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2004 Apr;29(4):302-5.
OBJECTIVE
To study influence of fungal elicitors on the biomass and ginseng saponin biosynthesis of hairy roots of Panax ginseng (HRPG).
METHOD
Fungal elicitors were extracted from Colletorichum lagnarinm, Phoma filtrate, Fusarium oxysponum, Asperillus niger and culture with HRPG. The total ginseng sponin and four kinds of monomeric sponins were analysed by UV-spectrophotometry and RP-HPLC.
RESULT
Fungal elicitors coula not only can influence on HRPG biomass and total ginseng sponin, but also improve or decrease some monomeric sponin. The total ginseng sponin could be increased to 3.649% but Rg1 and Re could not be detected when A. niger elicitors wss 20 mg x L(-1) in the culture fluid.
CONCLUSION
Fungal elicitor has specificity influence on secondary metabolite of HRPG. HRPG can biosynthesize specially active component by using specific fungal elicitor is used.
目的
研究真菌诱导子对人参毛状根（HRPG）生物量及人参皂苷生物合成的影响。
方法
从胶孢炭疽菌、茎点霉滤液、尖孢镰刀菌、黑曲霉中提取真菌诱导子，并与HRPG共培养。采用紫外分光光度法和反相高效液相色谱法分析总人参皂苷及4种单体皂苷。
结果
真菌诱导子不仅能影响HRPG生物量和总人参皂苷，还能提高或降低某些单体皂苷含量。当培养液中黑曲霉诱导子浓度为20mg·L⁻¹时，总人参皂苷含量可提高到3.649%，但未检测到Rg1和Re。
结论
真菌诱导子对HRPG次生代谢产物有特异性影响。利用特定的真菌诱导子，HRPG可生物合成特定活性成分。
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