Jeong Gwi-Taek, Park Don-Hee, Ryu Hwa-Won, Hwang Baik, Woo Je-Chang, Kim Doman, Kim Si-Wonk
Engineering Research Institute, Chonnam National University, Gwangju 500-757, Korea.
Appl Biochem Biotechnol. 2005 Spring;121-124:1147-57. doi: 10.1385/abab:124:1-3:1147.
Ginseng (Panax ginseng C.A. Meyer) hairy root cultures, established by infecting ginseng root discs with Agrobacterium rhizogenes, were used for secondary metabolite production. In this study, several elicitors [salicylic acid (SA), acetylsalicylic acid (ASA), yeast elicitor, and bacterial elicitor] were used to improve the productivity of useful metabolite in P. ginseng hairy root cultures. In SA elicitation, total ginseng saponin content increased slightly at lower elicitor dosages (0.1 to 0.5 mM). Also, the use of ASA as an elicitor resulted in the inhibition of biomass growth and an increase in total ginseng saponin content at every elicitor dosage (0.1 to 1.0 mM) by about 1.1 times. With yeast elicitor addition, hairy root growth was inhibited about 0.8-fold on a dry weight basis compared to the control, but total ginseng saponin content increased by about 1.17 times when compared to the control. The bacterial elicitor showed a slight inhibition of biomass growth, but total ginseng saponin content increased by about 1.23 times upon the addition of 1 mL.
通过用发根农杆菌感染人参根盘建立的人参（Panax ginseng C.A. Meyer）毛状根培养物用于次生代谢产物的生产。在本研究中，使用了几种诱导子[水杨酸（SA）、乙酰水杨酸（ASA）、酵母诱导子和细菌诱导子]来提高人参毛状根培养物中有用代谢产物的产量。在SA诱导中，较低诱导子剂量（0.1至0.5 mM）时人参总皂苷含量略有增加。此外，使用ASA作为诱导子导致在每个诱导子剂量（0.1至1.0 mM）下生物量生长受到抑制，人参总皂苷含量增加约1.1倍。添加酵母诱导子后，与对照相比，毛状根干重生长受到约0.8倍的抑制，但与人参总皂苷含量相比增加了约1.17倍。细菌诱导子对生物量生长有轻微抑制作用，但添加1 mL后，人参总皂苷含量增加了约1.23倍。
