Zhao M Q, Ding J Y, Liu J, Hu B
Institute of Biotechlogy, China Pharmaceutical University, Nanjing 210038 Jiangsu, China.
Zhongguo Zhong Yao Za Zhi. 2001 Dec;26(12):819-22.
To study basic conditions for biosynthesis arbutin by the hairy root of Panax ginseng.
Based on the content of arbutin and the biotransformation rate of hydroquione, these conditions such as the culture stage, the lasting time and the concentration of hydroquione fitting for biosynthesis were found.
After cultured for 22 days, transferred in fresh B5 medium having 2 mmol.L-1 hydroquione, the hairy root biotransformated hydroquione into arbutin in 24 hours, the bioconversion rate was 89.0% and the content of arbutin in dry root was 13.0%.
Arbutin, was which never found in the genus of Panax ginseng, can be biosynthesized by the hairy root of Panax ginseng.
研究人参毛状根生物合成熊果苷的基本条件。
根据熊果苷含量及对苯二酚的生物转化率，找出适合生物合成的培养阶段、持续时间及对苯二酚浓度等条件。
培养22天后，转入含2 mmol·L-1对苯二酚的新鲜B5培养基中，毛状根在24小时内将对苯二酚转化为熊果苷，生物转化率为89.0%，干根中熊果苷含量为13.0%。
人参属中从未发现过的熊果苷可由人参毛状根生物合成。
