[杂交平贝母鳞茎化学成分的研究]

[Studies on the chemical constituents from culbs of hybridized Bulbus Fritillariae Ussuriensis].

作者信息

Ruan Han-li, Zhang Yong-hui, Pan Xu-chu, Dong Tao, Wu Ji-zhou

机构信息

School of Pharmacy of Tongji Medical College, Huazhong University of Science And Technology Wuhan, 430030, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2004 Apr;29(4):331-4.

PMID:15706870

Abstract

OBJECTIVE

To study the chemical constituents of hybridized Bulbus Fritillariae Ussuriensis.

METHOD

The chemical constituents were isolated by silica column chromatography and their structures were identified by physical and chemical eveidences and spectral analysis (IR, 1H-NMR, 13C-NMR, 2D-NMR, MS).

RESULT

Seven compounds were obtained and identified as (20S,25S)5alpha, 14alpha, 17beta-cevanine-6beta-hydroxy-3-one (hupehenirine, ZF1), (20S,25S)5alpha, 14alpha, 17beta-cevanine-3beta-hydroxy-6-one (hupehenizine, ZF2), (20R,25S)5alpha, 14alpha-cevanine-3beta,20beta-dihydroxy-6-one (peiminine, verticinone, ZF3), (20S,25S)5alpha, 14alpha, 17beta-cevanine-3beta, 6beta-dihydroxy (hupehenine, ZF4), (20R,25S)5alpha, 14alpha-cevanine-3beta, 6beta, 20beta-trihydroxy (isoverticine, ZF5), (20R,25S)5alpha, 14alpha-cevanine-3beta, 6alpha, 20beta-trihydroxy (peimine, verticine, ZF6), (20S,25S)5alpha, 14alpha, 17beta-evanine-6beta-hydroxy-3beta-O-beta-D-glucoside (hupeheninoside, ZF7).

CONCLUSION

Compounds ZF1-7 were isolated from hybridized Bulbus Fritillariae Ussuriensis for the first time.

摘要

目的

研究杂交平贝母的化学成分。

方法

采用硅胶柱色谱法分离化学成分，并通过理化证据和光谱分析（红外光谱、1H-核磁共振、13C-核磁共振、二维核磁共振、质谱）鉴定其结构。

结果

获得7个化合物，分别鉴定为(20S,25S)5α,14α,17β-瑟文宁-6β-羟基-3-酮（湖贝甲素，ZF1）、(20S,25S)5α,14α,17β-瑟文宁-3β-羟基-6-酮（湖贝宁，ZF2）、(20R,25S)5α,14α-瑟文宁-3β,20β-二羟基-6-酮（浙贝母碱，贝母素乙，ZF3）、(20S,25S)5α,14α,17β-瑟文宁-3β,6β-二羟基（湖贝宁碱，ZF4）、(20R,25S)5α,14α-瑟文宁-3β,6β,20β-三羟基（异浙贝母碱，ZF5）、(20R,25S)5α,14α-瑟文宁-3β,6α,20β-三羟基（浙贝母碱，贝母素甲，ZF6）、(20S,25S)5α,14α,17β-埃瓦宁-6β-羟基-3β-O-β-D-葡萄糖苷（湖贝苷，ZF7）。