膜流化引发膜重塑，这会影响大肠杆菌的耐热性。

Membrane fluidization triggers membrane remodeling which affects the thermotolerance in Escherichia coli.

作者信息

Shigapova Natalia, Török Zsolt, Balogh Gábor, Goloubinoff Pierre, Vígh László, Horváth Ibolya

机构信息

Institute of Biochemistry, Biological Research Center of the Hungarian Academy of Sciences, P.O. Box 521, H-6701 Szeged, Hungary.

出版信息

Biochem Biophys Res Commun. 2005 Mar 25;328(4):1216-23. doi: 10.1016/j.bbrc.2005.01.081.

DOI:10.1016/j.bbrc.2005.01.081

PMID:15708006

Abstract

Treatment of Escherichia coli with non-lethal doses of heat or benzyl alcohol (BA) causes transient membrane fluidization and permeabilization, and induces the rapid transcription of heat-shock genes in a sigma32-dependent manner. This early response is followed by a rapid adaptation (priming) of the cells to otherwise lethal elevated temperature, in strong correlation with an observed remodeling of the composition and alkyl chain unsaturation of membrane lipids. The acquisition of cellular thermotolerance in BA-primed cells is unrelated to protein denaturation and is not accompanied by the formation of major heat-shock proteins, such as GroEL and DnaK. This suggests that the rapid remodeling of membrane composition is sufficient for the short-term bacterial thermotolerance.

摘要

用非致死剂量的热或苯甲醇（BA）处理大肠杆菌会导致细胞膜短暂的流动性增加和通透性改变，并以σ32依赖性方式诱导热休克基因的快速转录。这种早期反应之后是细胞对原本致死的高温的快速适应（预适应），这与观察到的膜脂成分和烷基链不饱和度的重塑密切相关。BA预适应细胞中获得的细胞耐热性与蛋白质变性无关，也不伴随着主要热休克蛋白（如GroEL和DnaK）的形成。这表明膜成分的快速重塑足以实现细菌的短期耐热性。