通过多重聚合酶链反应检测和鉴别类鼻疽伯克霍尔德菌、鼻疽伯克霍尔德菌和泰国伯克霍尔德菌。
Detection and differentiation of Burkholderia pseudomallei, Burkholderia mallei and Burkholderia thailandensis by multiplex PCR.
作者信息
Lee May-Ann, Wang Dongling, Yap Eu Hian
机构信息
Centre for BioMedical Sciences, Defence Medical and Environment Research Institute, DSO National Laboratories 27 Medical Drive, Singapore 117510, Singapore.
出版信息
FEMS Immunol Med Microbiol. 2005 Mar 1;43(3):413-7. doi: 10.1016/j.femsim.2004.10.008.
Burkholderia pseudomallei, a Gram-negative bacterium that causes melioidosis may be differentiated from closely related species of Burkholderia mallei that causes glanders and non-pathogenic species of Burkholderia thailandensis by multiplex PCR. The multiplex PCR consists of primers that flank a 10-bp repetitive element in B. pseudomallei and B. mallei amplifying PCR fragment of varying sizes between 400-700 bp, a unique sequence in B. thailandensis amplifying a PCR fragment of 308 bp and the metalloprotease gene amplifying a PCR fragment of 245 bp in B. pseudomallei and B. thailandensis. The multiplex PCR not only can differentiate the three Burkholderia species but can also be used for epidemiological typing of B. pseudomallei and B. mallei strains.
类鼻疽伯克霍尔德菌是一种可引发类鼻疽的革兰氏阴性菌，通过多重聚合酶链反应（multiplex PCR），它可与引发鼻疽的密切相关的鼻疽伯克霍尔德菌以及非致病性的泰国伯克霍尔德菌区分开来。该多重聚合酶链反应包含一些引物，这些引物位于类鼻疽伯克霍尔德菌和鼻疽伯克霍尔德菌中一段10个碱基对的重复元件两侧，可扩增出大小在400 - 700碱基对之间的不同聚合酶链反应片段；泰国伯克霍尔德菌中的一个独特序列可扩增出一个308碱基对的聚合酶链反应片段；金属蛋白酶基因在类鼻疽伯克霍尔德菌和泰国伯克霍尔德菌中可扩增出一个245碱基对的聚合酶链反应片段。这种多重聚合酶链反应不仅能够区分这三种伯克霍尔德菌，还可用于类鼻疽伯克霍尔德菌和鼻疽伯克霍尔德菌菌株的流行病学分型。
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