针对类鼻疽杆菌金属蛋白酶基因（mprA）的聚合酶链反应检测方法的开发与临床评估。
Development and clinical evaluation of a PCR assay targeting the metalloprotease gene (mprA) of B. pseudomallei.
作者信息
Neubauer H, Sprague L D, Joseph M, Tomaso H, Al Dahouk S, Witte A, Kinne J, Hensel A, Wernery R, Wernery U, Scholz H C
机构信息
Friedrich-Loeffler-Institut, Naumburger Str. 96a, D-07743 Jena, Germany.
出版信息
Zoonoses Public Health. 2007;54(1):44-50. doi: 10.1111/j.1863-2378.2007.01008.x.
A PCR assay targeting the metalloprotease gene (mprA) of Burkholderia pseudomallei was developed for the specific detection of this organism in pure cultures and clinical samples. All other closely related organisms including B. mallei the causative agent of glanders, and B. thailandensis tested negative. Burkholderia pseudomallei DNA was successfully amplified from paraffin-embedded lung tissue of a camel with a generalized B. pseudomallei infection. The developed PCR assay can be used as a simple tool for the specific and sensitive detection of B. pseudomallei.
开发了一种针对类鼻疽伯克霍尔德菌金属蛋白酶基因（mprA）的聚合酶链反应（PCR）检测方法，用于在纯培养物和临床样本中特异性检测该菌。所有其他密切相关的细菌，包括鼻疽的病原体鼻疽伯克霍尔德菌和泰国伯克霍尔德菌，检测结果均为阴性。从一只患有全身性类鼻疽伯克霍尔德菌感染的骆驼的石蜡包埋肺组织中成功扩增出类鼻疽伯克霍尔德菌DNA。所开发的PCR检测方法可作为一种简单工具，用于特异性和灵敏地检测类鼻疽伯克霍尔德菌。
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