Salehi Payam, Churchill Thomas A
Surgical-Medical Research Institute, 1074 Dentistry-Pharmacy Building, University of Alberta, Edmonton, Alta., Canada T6G 2N8.
Cryobiology. 2005 Feb;50(1):83-92. doi: 10.1016/j.cryobiol.2004.11.003. Epub 2005 Jan 22.
Donor nutritional status may be a determinant of small bowel (SB) quality following storage. In this study, we investigated the effect of donor nutritional status and a proven nutrient-rich preservation solution on graft quality following cold storage.
Rats were fasted (12-14 h) or non-fasted. SB (n=6) was flushed vascularly with modified University of Wisconsin (UW) solution and flushed luminally with UW or an amino acid-rich (AA) solution as follows: Fasted. UWV, none; UWL, UW solution; AAL, AA solution. Non-fasted. UWV, none; UWL, UW solution; AAL, AA solution. Energetics, peroxidation (malondialdehyde; MDA), glutathione and histology were assessed over 24 h at 4 degrees C.
Energetics (ATP, ATP/ADP, and energy charge) were significantly higher in AAL (fasted and non-fasted) groups than other groups. However, there were no differences in energetics parameters between fasted and non-fasted animals in all groups. MDA was higher in fasted groups than non-fasted tissues; interestingly, AAL values were up to 10-fold lower than other groups. Higher glutathione levels were detected in non-fasted AAL tissues. Mucosal integrity was markedly superior in luminally treated tissues (UWL and AAL) in fasted and non-fasted states. Most noteably, AAL tissues from fasted animals exhibited grade 2 injury (villus clefting), whereas normal mucosa was observed in non-fasted tissues (grade 0).
Luminal flushing and a nutrient-rich preservation solution improve energetics, oxidative stress, and mucosal integrity during storage. Poorer donor nutritional status does not affect energetics throughout storage, but causes mucosal injury as a result of increased oxidative stress, even after a brief period of donor fasting.
供体营养状况可能是储存后小肠（SB）质量的一个决定因素。在本研究中，我们调查了供体营养状况和一种经证实的富含营养的保存液对冷藏后移植物质量的影响。
将大鼠禁食（12 - 14小时）或不禁食。对小肠（n = 6）进行血管冲洗，用改良的威斯康星大学（UW）溶液，并用UW溶液或富含氨基酸（AA）的溶液进行肠腔冲洗，如下：禁食组。UWV，无；UWL，UW溶液；AAL，AA溶液。非禁食组。UWV，无；UWL，UW溶液；AAL，AA溶液。在4℃下24小时内评估能量代谢、过氧化（丙二醛；MDA）、谷胱甘肽和组织学情况。
AAL组（禁食和非禁食）的能量代谢（ATP、ATP/ADP和能量电荷）显著高于其他组。然而，所有组中禁食和非禁食动物的能量代谢参数没有差异。禁食组的MDA高于非禁食组织；有趣的是，AAL组的值比其他组低至10倍。在非禁食AAL组织中检测到更高的谷胱甘肽水平。在禁食和非禁食状态下，肠腔处理的组织（UWL和AAL）的黏膜完整性明显更好。最值得注意的是，禁食动物的AAL组织表现出2级损伤（绒毛裂开），而在非禁食组织中观察到正常黏膜（0级）。
肠腔冲洗和富含营养的保存液可改善储存期间的能量代谢、氧化应激和黏膜完整性。供体营养状况较差在整个储存过程中不影响能量代谢，但会因氧化应激增加导致黏膜损伤，即使供体短暂禁食后也是如此。
