Balducci L, Hardy C L
University of Mississippi Medical Center, Jackson.
Pathobiology. 1992;60(2):68-71. doi: 10.1159/000163700.
During the progression of Lewis lung carcinoma (LLC) in C57Bl/6 mice, a neutrophilia with granulocytosis develops. This heightened myeloid response is further reflected in a progressively increasing concentration of precursors CFU-GM (colony-forming unit, granulocyte-macrophage) in the marrow. However, during the final days of tumor growth (day 19) the cycling fraction of CFU-GM declines in LLC mice, although their concentration remains high. It was of interest to explore in the present study whether this decline in proliferation of committed myeloid progenitors was due to exhaustion of the pool of early and late pluripotential stem cells CFU-S (colony-forming unit, spleen). The results indicate that early stem cells experience an initial proliferative burst (day 14) during tumor growth, but fail to replenish their own pool by day 19, suggesting that their self-replicative ability may become exhausted during growth of this cancer. The implications of these findings to conditions of chronic hemopoietic stress are discussed.
在C57Bl/6小鼠的Lewis肺癌（LLC）进展过程中，会出现伴有粒细胞增多的中性粒细胞增多症。这种增强的髓系反应进一步体现在骨髓中前体CFU-GM（集落形成单位，粒细胞-巨噬细胞）的浓度逐渐增加。然而，在肿瘤生长的最后几天（第19天），LLC小鼠中CFU-GM的循环分数下降，尽管其浓度仍然很高。在本研究中，探究定向髓系祖细胞增殖的这种下降是否是由于早期和晚期多能干细胞CFU-S（集落形成单位，脾）池的耗竭，这一点很有意义。结果表明，早期干细胞在肿瘤生长期间经历了初始增殖爆发（第14天），但到第19天未能补充自身池，这表明它们的自我复制能力可能在这种癌症生长期间耗尽。讨论了这些发现对慢性造血应激状况的影响。
