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Construction of chimeric antibodies: cloning of immunoglobulin genes including their promoter regions by PCR.

作者信息

Mocikat R, Kütemeier G, Harloff C

机构信息

GSF-Institut für Immunologie, München, FRG.

出版信息

Biotechniques. 1992 Mar;12(3):355-6.

PMID:1571141
Abstract

In the production of recombinant antibodies, it is necessary to have an immunoglobulin gene promoter for driving the expression of the antibody genes. Here we describe a simple PCR method that allows cloning of the immunoglobulin genes together with their own promoters despite the fact that the sequence of the upstream part of the gene is unknown.

摘要

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