氨苄青霉素和亚硝酸盐诱导的β-内酰胺酶I失活的可逆性
Reversibility of the ampicillin-and nitrite-induced inactivation of beta-lactamase I.
作者信息
Durkin J P, Dmitrienko G I, Viswanatha T
出版信息
Can J Biochem. 1977 Apr;55(4):453-7. doi: 10.1139/o77-063.
DOI:10.1139/o77-063
PMID:15712
Abstract
beta-Lactamase I was isolated from Bacillus cereus 569/H. Treatment with ampicillin in the presence of sodium nitrite at pH 4 or 5 resulted in the inactivation of the enzyme presumably by modification of a carboxyl group in the active site. However, this inactivation was rapidly, reversible at neutral pH and the available evidence points to the participation of a second carboxyl group which is involved in the reactivation process.
摘要
β-内酰胺酶I是从蜡样芽孢杆菌569/H中分离出来的。在pH值为4或5的亚硝酸钠存在下用氨苄青霉素处理，可能是通过修饰活性位点中的羧基导致该酶失活。然而，这种失活在中性pH值下迅速且可逆，现有证据表明有第二个羧基参与了再激活过程。
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