Vicario María, Crespí Mar, Franch Angels, Amat Concepció, Pelegrí Carme, Moretó Miquel
Departament de Fisiologia, Facultat de Farmàcia, Universitat de Barcelona, Av. de Joan XXIII s/n, E-08028 Barcelona, Spain.
Dig Dis Sci. 2005 Jan;50(1):143-50.
Models using dextran sulfate sodium (DSS) to induce experimental colitis in rodents have been performed mostly in adult animals. For this reason, we aimed to develop a model of colitis in young rats. DSS was administered to 30-day-old rats at concentrations ranging from 0.5 to 5% in drinking water. Young rats were remarkably sensitive to DSS since clinical symptoms rapidly rose with 5% DSS and most animals died after the fifth day. With 1 and 2% DSS, the severity of mucosal lesions was also high on day 7, the animals showing leukocytosis and anemia. At 0.5% DSS, leukocytosis and mild colonic lesions were induced. This concentration of DSS significantly increased myeloperoxidase activity and goblet cell number in the colon, indicating mucosal inflammation. Since food consumption was not reduced by 0.5% DSS, we suggest that this protocol can be used to study the effects of dietary supplements on intestinal inflammatory processes.
使用葡聚糖硫酸钠（DSS）诱导啮齿动物实验性结肠炎的模型大多是在成年动物身上进行的。因此，我们旨在建立一种幼鼠结肠炎模型。将DSS以0.5%至5%的浓度给予30日龄大鼠，让其饮用含药饮水。幼鼠对DSS非常敏感，因为饮用5% DSS后临床症状迅速出现，大多数动物在第五天后死亡。给予1%和2% DSS时，第7天黏膜损伤的严重程度也很高，动物出现白细胞增多和贫血。给予0.5% DSS时，会诱导白细胞增多和轻度结肠损伤。这种浓度的DSS显著增加了结肠中的髓过氧化物酶活性和杯状细胞数量，表明存在黏膜炎症。由于0.5% DSS并未使食物摄入量减少，我们认为该方案可用于研究膳食补充剂对肠道炎症过程的影响。
