Lara-Villoslada Federico, Debras Elisabeth, Nieto Ana, Concha Angel, Gálvez Julio, López-Huertas Eduardo, Boza Julio, Obled Christiane, Xaus Jordi
Department of Immunology and Animal Sciences. PULEVA BIOTECH, S.A. Camino de Purchil no. 66, 18004 Granada, Spain.
Clin Nutr. 2006 Jun;25(3):477-88. doi: 10.1016/j.clnu.2005.11.004. Epub 2006 Jan 10.
There is increased interest in the study of manipulation of the flora with pro- and prebiotics regarding inflammatory bowel disease. The aim of this work was to evaluate the effect of oligosaccharides from goat milk in a rat model of dextran sodium sulfate- (DSS-) induced colitis.
Twenty rats were fed the same diet but with different sources of fiber (5% of the diet): cellulose or a mixture of goat's milk oligosaccharides (GMO) and cellulose. DSS treatment was used to induce a colonic inflammation. Several clinical and inflammatory parameters, as well as intestinal micorbiota and gene expression by DNA microarray technology, were evaluated.
DSS induced a decrease in body weight which was not observed in rats fed the GMO (decrease of 21+/-11% in control rats vs increase of 5.2+/-8.6 in GMO rats, P<0.05). DSS also caused an acute colonic inflammatory process which was weaker in rats fed the GMO, as shown by colon myeloperoxidase activity (0.53+/-0.16 vs 0.14+/-0.07U/mg of protein, P<0.05), as well as clinical symptoms measured by a scoring system (1.25+/-1.14 vs 0.4+/-0.07, P<0.05). GMO rats also showed less severe colonic lesions and a more favorable intestinal microbiota. The expression of genes involved in intestinal function, such as mucine-3, was down-regulated in DSS-control rats but returned to normal values in GMO rats.
GMO reduce intestinal inflammation and contribute to the recovery of damaged colonic mucosa.
关于炎症性肠病，使用益生元和益生菌对菌群进行调控的研究越来越受到关注。本研究的目的是评估山羊乳寡糖在葡聚糖硫酸钠（DSS）诱导的大鼠结肠炎模型中的作用。
20只大鼠喂食相同的饮食，但含有不同来源的纤维（占饮食的5%）：纤维素或山羊乳寡糖（GMO）与纤维素的混合物。采用DSS处理诱导结肠炎症。评估了几个临床和炎症参数，以及肠道微生物群和通过DNA微阵列技术检测的基因表达。
DSS导致体重下降，而喂食GMO的大鼠未出现体重下降（对照大鼠体重下降21±11%，而GMO大鼠体重增加5.2±8.6%，P<0.05）。DSS还引发了急性结肠炎症过程，喂食GMO的大鼠炎症较轻，如结肠髓过氧化物酶活性所示（0.53±0.16对0.14±0.07U/mg蛋白质，P<0.05），以及通过评分系统测量的临床症状（1.25±1.14对0.4±0.07，P<0.05）。GMO大鼠的结肠病变也较轻，肠道微生物群更有利。参与肠道功能的基因，如粘蛋白-3的表达，在DSS对照大鼠中下调，但在GMO大鼠中恢复到正常水平。
GMO可减轻肠道炎症，有助于受损结肠黏膜的恢复。
