Illyés G, Hamar J
National Institute of Traumatology, Budapest, Hungary.
Int J Exp Pathol. 1992 Apr;73(2):161-72.
Previous studies have shown serious mucosal damage and destruction to be associated with intestinal ischaemia/reperfusion. As both destruction and concomitant regeneration can be observed together in this potentially lethal condition we have studied the development and sequence of events by evaluating morphological changes of the small intestine in an ischaemia/reperfusion model in anaesthetized rats. Forty-five minutes of ischaemia was followed by 4 hours of reperfusion. Tissue samples of the small intestine were examined by light microscopy in normal and semithin sections. Samples were collected at the end of ischaemia, at 10 min, and at 1, 2, 3 and 4 hours of reperfusion, respectively. Survival was assessed in a parallel group of anaesthetized rats. The morphological changes were described and they were analysed by a semi-quantitative method using five different markers of histological alteration. The mortality rate of a control survival group was 100%. Mucosal destruction at the end of ischaemia and during reperfusion was diffuse and steadily increased as a function of reperfusion time. At the same time the epithelium showed intensive regenerative growth which covered the denuded mucosal surface by the third hour of reperfusion. A secondary epithelial desquamation followed this process and was accompanied by heavy inflammatory cell infiltration. The infiltration may be the cause of the secondary epithelial injury.
先前的研究表明，严重的黏膜损伤和破坏与肠道缺血/再灌注有关。在这种潜在致命的情况下，破坏和伴随的再生可同时观察到，因此我们通过评估麻醉大鼠缺血/再灌注模型中小肠的形态变化，研究了事件的发展过程和顺序。缺血45分钟后再灌注4小时。分别在缺血结束时、再灌注10分钟时以及再灌注1、2、3和4小时时，采集小肠组织样本，制作正常和半薄切片，通过光学显微镜进行检查。在另一组平行的麻醉大鼠中评估存活率。描述形态学变化，并使用五种不同的组织学改变标记物，通过半定量方法进行分析。对照组的死亡率为100%。缺血结束时和再灌注期间的黏膜破坏是弥漫性的，并随着再灌注时间的延长而稳步增加。与此同时，上皮细胞呈现强烈的再生性生长，在再灌注3小时时覆盖了裸露的黏膜表面。在此过程之后，出现了继发性上皮脱屑，并伴有大量炎性细胞浸润。这种浸润可能是继发性上皮损伤的原因。
