Zhang Lin, You Jinmao, Ping Guichen, Zhang Weibing, Yan Chao, Zhang Yukui
Dalian Institute of Chemical Physics, The Chinese Academy of Sciences, Dalian 116011, China.
Se Pu. 2004 Mar;22(2):166-9.
2-(9-Carbazole)-ethyl chloroformate (CEOC) has been used for the pre-column derivatization of amino acids in micellar electrokinetic capillary chromatography (MEKC). Nine amino acid standards (Ser, Thr, Gly, Glu, Asp, Val, Ile, Leu, Phe) were labeled with CEOC and separated in a buffer system containing 20 mmol/L borate-30 mmol/L sodium lauryl sulfate (SDS) and 3% acetonitrile at pH 9.0 with an applied voltage of 18 kV and column temperature of 25 degrees C. The derivatives were detected at UV 214 nm. Under optimal conditions, this method offers a baseline separation for nine CEOC-derivatized amino acids within 14 min. The linearity range was from 0.025 to 0.25 mmol/L and the detection limits were from 2.15 to 2.46 micromol/L.
2-（9-咔唑基）-氯甲酸乙酯（CEOC）已用于胶束电动毛细管色谱法（MEKC）中氨基酸的柱前衍生化。九种氨基酸标准品（丝氨酸、苏氨酸、甘氨酸、谷氨酸、天冬氨酸、缬氨酸、异亮氨酸、亮氨酸、苯丙氨酸）用CEOC进行标记，并在含有20 mmol/L硼酸盐-30 mmol/L十二烷基硫酸钠（SDS）和3%乙腈的缓冲体系中，于pH 9.0、施加电压18 kV及柱温25℃条件下进行分离。衍生物于紫外214 nm处检测。在最佳条件下，该方法可在14分钟内对九种CEOC衍生化氨基酸实现基线分离。线性范围为0.025至0.25 mmol/L，检测限为2.15至2.46 μmol/L。
