Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), Department of Chemistry, Wuhan University, Wuhan, China.
Anal Bioanal Chem. 2011 Jul;401(1):297-304. doi: 10.1007/s00216-011-5056-6. Epub 2011 May 15.
An effective micellar electrokinetic capillary chromatography with laser-induced fluorescence detection (MEKC-LIF) method has been proposed for the separation and the determination of 16 amino acids and two catecholamines using a new fluorogenic reagent, 3-(4-chlorobenzoyl)-2-quinolinecarboxaldehyde (Cl-BQCA), as the derivatizing reagent. The highest derivatization efficiency was achieved in pH 8.0 borate buffer at 50 °C for 50 min. The optimal separation of Cl-BQCA-labeled amines was obtained with a running buffer (pH 9.15) containing 120 mM boric acid, 38.5 mM sodium dodecyl sulfate, and 17% acetonitrile. The detection limit (S/N = 3) was found to be as low as 1.4 nM. The present method has been successfully used to detect amino acids and catecholamines in HEK293 and PC12 cell samples. This study explores the potential of MEKC-LIF with Cl-BQCA labeling as a tool for monitoring amino acids and catecholamines during the complex physiological and behavioral processes in various matrices.
一种有效的胶束电动毛细管色谱与激光诱导荧光检测（MEKC-LIF）方法已被提出，用于分离和测定 16 种氨基酸和两种儿茶酚胺，使用一种新的荧光衍生试剂，3-（4-氯苯甲酰基）-2-喹啉甲醛（Cl-BQCA）作为衍生试剂。在 50°C 的 pH 8.0 硼酸盐缓冲液中，衍生效率最高，时间为 50 分钟。在含有 120mM 硼酸、38.5mM 十二烷基硫酸钠和 17%乙腈的运行缓冲液（pH 9.15）中，可获得最佳的 Cl-BQCA 标记胺的分离效果。检测限（S/N=3）低至 1.4 nM。本方法已成功用于检测 HEK293 和 PC12 细胞样品中的氨基酸和儿茶酚胺。本研究探讨了 MEKC-LIF 与 Cl-BQCA 标记联用作为监测各种基质中复杂生理和行为过程中氨基酸和儿茶酚胺的工具的潜力。
