Zhao Shan, Wu Danan, Wang Peng
Beijing Center for Disease Prevention and Control, Beijing 100013, China.
Se Pu. 2004 May;22(3):267-9.
An efficient method for analyzing seven sexual hormones in cosmetics, namely, estriol, estradiol-17beta, estrone, testosterone, methyl-testosterone, progesterone and diethylstilbestrol by reversed-phase high performance liquid chromatography was developed. Samples were saponified with 20 g/L sodium hydroxide. The sexual hormones were then extracted with dichloromethane-acetic acetate (40:1, v/v) under acidic conditions (pH 3, adjusted with 1 mol/L HCl). An XTerra RP18 column was employed and a mixture of water-methanol-acetonitrile (50:32:18, v/v) was used as mobile phase. The seven sexual hormones were detected at 230 nm. The average spiked recoveries for the seven sexual hormones ranged from 75.6% to 97.8% with relative standard deviations of 1.9% to 7.2%. The linear ranges of determination were from 5 to 50 mg/L with correlation coefficients of 0.9999, and the limits of detection were from 3.7 to 12 ng. The method is suitable to simultaneously assay the seven sexual hormones in cosmetics with simplicity and accuracy in a single injection.
建立了一种用反相高效液相色谱法分析化妆品中七种性激素的有效方法，这七种性激素分别是雌三醇、17β-雌二醇、雌酮、睾酮、甲基睾酮、孕酮和己烯雌酚。样品用20 g/L氢氧化钠皂化。然后在酸性条件（用1 mol/L盐酸调至pH 3）下用二氯甲烷 - 乙酸乙酯（40:1，v/v）萃取性激素。采用XTerra RP18柱，以水 - 甲醇 - 乙腈（50:32:18，v/v）的混合物作为流动相。在230 nm处检测这七种性激素。七种性激素的平均加标回收率在75.6%至97.8%之间，相对标准偏差为1.9%至7.2%。测定的线性范围为5至50 mg/L，相关系数为0.9999，检测限为3.7至12 ng。该方法适用于在一次进样中简单、准确地同时测定化妆品中的七种性激素。
