Huang J, Asaeda N, Takeuchi Y, Shibata E, Hisanaga N, Ono Y, Kato K
Department of Hygiene, Nagoya University School of Medicine, Japan.
Br J Ind Med. 1992 Apr;49(4):282-6. doi: 10.1136/oem.49.4.282.
The dose dependent effects of chronic exposure to toluene on the neuronal marker proteins (gamma-enolase, calbindin-D28k) and glial cell marker proteins (alpha-enolase, creatine kinase-B, and beta-S100 protein) were investigated in the central nervous system (CNS) of rats. Three groups of animals were exposed to 100 ppm, 300 ppm, or 1000 ppm toluene vapour eight hours a day, six days a week for 16 weeks. The contents of the marker substances were determined with enzyme immunoassays. A significant increase in the three glial cell marker proteins was noted in the cerebellum after exposure to 100 ppm toluene; a more pronounced increase occurred at the higher toluene concentrations. beta-S100 protein also exhibited a dose dependent increase in the brainstem and spinal cord. On the other hand, the two neuronal cell markers did not show a quantitative decrease in the CNS. This means that the development of gliosis, rather than neurone death, is induced by chronic exposure to toluene. The significant biochemical changes induced around the threshold limit value and the concentration dependent alterations suggest that these nerve specific marker proteins may be used to evaluate solvent related damage to the CNS.
研究了大鼠中枢神经系统（CNS）中长期暴露于甲苯对神经元标记蛋白（γ-烯醇化酶、钙结合蛋白-D28k）和神经胶质细胞标记蛋白（α-烯醇化酶、肌酸激酶-B和β-S100蛋白）的剂量依赖性影响。三组动物每天8小时、每周6天暴露于100 ppm、300 ppm或1000 ppm的甲苯蒸气中，持续16周。用酶免疫测定法测定标记物质的含量。暴露于100 ppm甲苯后，小脑内三种神经胶质细胞标记蛋白显著增加；在较高甲苯浓度下增加更为明显。β-S100蛋白在脑干和脊髓中也呈现剂量依赖性增加。另一方面，两种神经元细胞标记物在中枢神经系统中未显示出定量减少。这意味着慢性暴露于甲苯会诱发神经胶质增生，而非神经元死亡。在阈限值附近诱导的显著生化变化以及浓度依赖性改变表明，这些神经特异性标记蛋白可用于评估溶剂对中枢神经系统的相关损害。
