José Marta, Gajardo Rodrigo, Jorquera Juan I
Research and Development Area, Instituto Grifols, S.A., Poligon Llevant, C/Can Guasch, 2, 08150-Parets del Vallès, Barcelona, Spain.
Biologicals. 2005 Mar;33(1):9-16. doi: 10.1016/j.biologicals.2004.10.003.
The implementation of nucleic acid amplification technology (NAT) for detection of HCV, HIV-1 and HBV has undoubtedly contributed to the viral safety of blood, reducing the window period. One important matter related to the stability of RNA/DNA is the effect of the storage conditions on samples. In a previous work, we studied the stability of HCV RNA in plasma samples after storage at different temperatures. This work is an update on the follow-up of a sample containing 100 IU/ml HCV RNA for 5 years at -20 degrees C, showing no decrease in the initial titre. The nucleic acid stability of other viruses, such as HIV-1 and HBV, has also been studied. At -20 degrees C, samples containing HIV-1 were followed up for approximately 3 years and the results obtained show no decay in HIV-1 RNA detectability. Regardless of the HIV-1 RNA concentration, samples stored at 5 degrees C maintain their titre for at least 14 days. At 25 degrees C, the HIV-1 RNA half-life was determined at nearly 7 days. The HBV DNA, at 5 degrees C and 25 degrees C, is stable for at least 28 days, regardless of the initial titre.
采用核酸扩增技术（NAT）检测丙型肝炎病毒（HCV）、人类免疫缺陷病毒1型（HIV-1）和乙型肝炎病毒（HBV）无疑有助于提高血液的病毒安全性，缩短窗口期。与RNA/DNA稳定性相关的一个重要问题是储存条件对样本的影响。在之前的一项研究中，我们研究了血浆样本中HCV RNA在不同温度下储存后的稳定性。这项工作是对一份含有100 IU/ml HCV RNA的样本在-20℃下进行5年跟踪的更新，结果显示初始滴度没有下降。其他病毒，如HIV-1和HBV的核酸稳定性也已得到研究。在-20℃下，对含有HIV-1的样本进行了约3年的跟踪，结果显示HIV-1 RNA的可检测性没有下降。无论HIV-1 RNA浓度如何，储存在5℃的样本至少14天内保持其滴度。在25℃下，HIV-1 RNA的半衰期测定为近7天。无论初始滴度如何，HBV DNA在5℃和25℃下至少28天内保持稳定。
