埃塞俄比亚亚的斯亚贝巴圣保罗医院千年医药学院样本管理对定量 HIV-1 病毒载量测量的影响。
Effect of sample management on quantitative HIV-1 viral load measurement at Saint Paul's Hospital Millennium Medical College, Addis Ababa, Ethiopia.
机构信息
HIV/AIDS Disease Research Team, TB and HIV/AIDS Disease Research Directorate, Ethiopian Public Health Institute, Addis Ababa, Ethiopia.
Department of Medical Laboratory Sciences, College of Health Sciences, Addis Ababa University, Addis Ababa, Ethiopia.
出版信息
PLoS One. 2022 Jun 14;17(6):e0269943. doi: 10.1371/journal.pone.0269943. eCollection 2022.
PURPOSE
This study was meant to determine the effect of time to plasma separation, storage duration, freeze-thawing cycle and dilution proportion on the HIV-1 viral load level.
METHODS
Experimental study design was employed by collecting 10mL whole blood samples into two EDTA tubes from 88 eligible HIV infected patients at St Paul's Hospital Millennium Medical College. The viral load test was done using Abbott m2000sp/rt analyzer. Data was entered into Microsoft excel and analyzed by SPSS version 20. Repeated measure analysis of variance was used to compare HIV RNA viral load mean difference between different time to plasma separation, storage, freeze-thawing cycles and dilution levels. Post-hoc analysis was employed to locate the place of significant differences. P value less than 0.05 was used to declare statistical significance while viral RNA level of 0.5 log copies/ml was used to determine clinical significance.
RESULTS
There was significant HIV-1 RNA viral load log mean difference between plasma separation time at 6 hours (hrs) and 24hrs (p<0.001). There was also significant HIV-1 RNA viral load log mean difference between plasma tested within 6hrs and those stored at 2-8°C for 15 days (p = 0.006), and between plasma stored at 2-8°C for 6 days versus 15 days (p<0.001). There was significant log mean difference between plasma that was exposed to fourth cycle of freeze-thawing after storage at -20°C when compared with plasma tested within 6hrs (p = 0.013).
CONCLUSION
Plasma separated at 24hrs, stored at 2-8°C for 15 days or freeze-thawed for four cycles had significant effect on HIV viral load level. However, the differences were not clinically significant at a cut-off viral load level of 0.5 log copies/ml. Avoiding delays to plasma separation beyond 24 hrs, storing at 2-8°C for 15 days and freeze-thawing for no more than 4 cycles is recommended to improve the result quality.
目的
本研究旨在确定血浆分离时间、储存时间、冻融循环和稀释比例对 HIV-1 病毒载量水平的影响。
方法
采用实验研究设计，从圣保罗医院千年医科大学的 88 名符合条件的 HIV 感染患者中采集 10mL 全血样本到两支 EDTA 管中。使用 Abbott m2000sp/rt 分析仪进行病毒载量检测。数据输入 Microsoft excel 并使用 SPSS 版本 20 进行分析。采用重复测量方差分析比较不同血浆分离时间、储存、冻融循环和稀释水平之间 HIV RNA 病毒载量平均值的差异。采用事后分析定位显著差异的位置。P 值小于 0.05 表示具有统计学意义，而病毒 RNA 水平为 0.5 log 拷贝/ml 用于确定临床意义。
结果
血浆分离时间在 6 小时（hrs）和 24 小时（p<0.001）之间存在显著的 HIV-1 RNA 病毒载量对数均值差异。在 6 小时内检测的血浆与在 2-8°C 储存 15 天的血浆之间（p = 0.006）以及在 2-8°C 储存 6 天与 15 天的血浆之间（p<0.001）也存在显著的 HIV-1 RNA 病毒载量对数均值差异。与在 6 小时内检测的血浆相比，在-20°C 下储存并经历第四次冻融循环的血浆存在显著的对数均值差异（p = 0.013）。
结论
血浆分离时间超过 24 小时、在 2-8°C 下储存 15 天或冻融循环 4 次对 HIV 病毒载量水平有显著影响。然而，在 0.5 log 拷贝/ml 的截止病毒载量水平下，差异无临床意义。建议避免血浆分离延迟超过 24 小时，在 2-8°C 下储存 15 天，并避免冻融循环超过 4 次，以提高结果质量。
Zotero 插件