Dutta Ajaswrata, Batra Jyoti, Pandey-Rai Sashi, Singh Digvijay, Kumar Sushil, Sen Jayanti
National Centre for Plant Genome Research, Jawaharlal Nehru University Campus, P.O. Box 10531, 110 067 New Delhi, India.
Planta. 2005 Jan;220(3):376-83. doi: 10.1007/s00425-004-1380-9. Epub 2004 Nov 10.
Madagascar periwinkle, Catharanthus roseus (L.) G. Don, a medicinally important plant, produces anticancer dimeric alkaloids, vinblastine and vincristine, in the leaves and accumulates antihypertensive alkaloids, ajmalicine and serpentine, in the roots. This plant grows wild in distant tropical and sub-tropical geographical locations with different agro-climates and shows wide variations in morphological and alkaloid yield-related traits. In order to understand the correlation between the expression of terpenoid indole alkaloid (TIA) pathway genes and accumulation of related alkaloids, six different genetic resources of C. roseus, including the medicinal cultivars Nirmal, Prabal, Dhawal, the mutants gsr-3 and gsr-6, and one horticultural variety, Pacifica blush, were studied. The expression profiles of one early and two late TIA biosynthetic pathway genes, namely, strictosidine synthase, desacetoxyvindoline 4-hydroxylase and deacetyl vindoline 4-O-acetyl transferase were analyzed in these plants. A positive correlation between transcript abundance and accumulation of related alkaloids was observed in the different genetic resources. The potential of these TIA biosynthetic pathway genes for use in screening of high-yielding C. roseus germplasm has been discussed.
马达加斯加长春花（Catharanthus roseus (L.) G. Don）是一种具有重要药用价值的植物，其叶片中产生抗癌二聚体生物碱长春碱和长春新碱，根部积累降压生物碱阿吗碱和蛇根碱。这种植物在遥远的热带和亚热带地理位置野生生长，具有不同的农业气候条件，并且在形态和生物碱产量相关性状上表现出广泛的变异。为了了解萜类吲哚生物碱（TIA）途径基因的表达与相关生物碱积累之间的相关性，对六种不同的长春花遗传资源进行了研究，包括药用品种Nirmal、Prabal、Dhawal，突变体gsr - 3和gsr - 6，以及一个园艺品种Pacifica blush。分析了这些植物中一个早期和两个晚期TIA生物合成途径基因的表达谱，即裂环马钱子苷合酶、去乙酰氧基长春花碱4 - 羟化酶和去乙酰长春花碱4 - O - 乙酰转移酶。在不同的遗传资源中观察到转录本丰度与相关生物碱积累之间存在正相关关系。讨论了这些TIA生物合成途径基因在筛选高产长春花种质中的应用潜力。
