Huang Hsi-Ya, Chiu Chen-Wen, Chen Yen-Chen, Yeh Jui-Ming
Department of Chemistry and Center for Nanotechnology at CYCU, Chung Yuan Christian University, Chung-Li, Taiwan, R.O.C.
Electrophoresis. 2005 Feb;26(4-5):895-902. doi: 10.1002/elps.200410117.
Microemulsion electrokinetic chromatography (MEEKC) and micellar electrokinetic chromatograpy (MEKC) were compared for their abilities to separate and detect ten similar benzophenones, which are commonly used as UV filters in various plastic and cosmetic products. Sodium dodecyl sulfate (SDS) concentration and column temperature rarely affected separation resolution for MEEKC, but separation of benzophenones could be improved by changing the SDS concentration and column temperature for MEKC. Buffer pH and ethanol (organic modifier) were found to markedly influence the separation selectivity for both MEEKC and MEKC systems. In addition, a higher electric voltage improved the separation efficiency without a noticeable reduction in separation resolution for MEEKC, whereas it caused a poor separation resolution for the MEKC system.
比较了微乳液电动色谱法（MEEKC）和胶束电动色谱法（MEKC）分离和检测十种类似二苯甲酮的能力，这些二苯甲酮常用于各种塑料和化妆品中作为紫外线过滤剂。十二烷基硫酸钠（SDS）浓度和柱温对MEEKC的分离分辨率影响很小，但通过改变MEKC的SDS浓度和柱温可以改善二苯甲酮的分离效果。发现缓冲液pH值和乙醇（有机改性剂）对MEEKC和MEKC系统的分离选择性均有显著影响。此外，较高的电压提高了MEEKC的分离效率，且分离分辨率没有明显降低，而对MEKC系统则导致分离分辨率较差。
