[大蒜素对人巨细胞病毒即刻早期抗原-IE72和IE86在人胚肺细胞中表达的影响]
[Effects of allitridin on the expression of human cytomegalovirus immediate early antigens-IE72 and IE86 in human embryonic lung cells].
作者信息
Zhen Hong, Fang Feng, Liu Zhi-Feng, Nie Xing-Cao, Cui Wen, Li Ge
机构信息
Pediatric Department, Tongji Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.
出版信息
Zhongguo Zhong Yao Za Zhi. 2005 Jan;30(1):47-9.
OBJECTIVE
To investigate the effect of allitridin injection on the expression of human cytomegalovirus (HCMV) immediate-early antigens (IEAs including IE72 and IE86) in human embryonic lung cells.
METHOD
HCMV AD 169 Virus strain infected cell model (MOI = 2.5 and 0.25, respectively) were established, and then treated with ICm5 and MTC doses of allitridin. Western blot was used to analyze the of IE72 and IE86 expression after the treatment, ganciclovir(GCV, IC50 and 2.3 x IC50) treatment as control.
RESULT
No matter what kind of MOI was used, both IE86 and IE72 antigens' expression was effectively suppressed by allitridin treatment, and the inhibitory rate of IE86 was almost twice of IE72's. Compared with GCV, allitridin had stronger inhibitory effect on IE86 expressing, although its efficacy on IE72 was weaker than GCV.
CONCLUSION
Allitridin could suppress the expression of IE72 and IE86, especially for IE86 expressing, maybe it is ore of key role in the mechanism of allitridin against HCMV.
目的
研究大蒜素注射液对人胚肺细胞中人巨细胞病毒（HCMV）即刻早期抗原（包括IE72和IE86的IEA）表达的影响。
方法
建立HCMV AD169病毒株感染细胞模型（MOI分别为2.5和0.25），然后用大蒜素的ICm5和MTC剂量进行处理。用Western blot分析处理后IE72和IE86的表达情况，以更昔洛韦（GCV，IC50和2.3×IC50）处理作为对照。
结果
无论使用何种MOI，大蒜素处理均能有效抑制IE86和IE72抗原的表达，且IE86的抑制率几乎是IE72的两倍。与GCV相比，大蒜素对IE86表达的抑制作用更强，尽管其对IE72的疗效弱于GCV。
结论
大蒜素可抑制IE72和IE86的表达，尤其是对IE86的表达，这可能是大蒜素抗HCMV机制中的关键作用之一。
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