[大蒜素对人巨细胞病毒即刻早期、早期和晚期基因体外转录的影响]

[Effects of allitridin on transcription of immediate-early, early and late genes of human cytomegalovirus in vitro].

作者信息

Zhang Ju, Xiang Zhidan, Liu Xinglou, Wang Hui, Li Ge, Fang Feng

机构信息

Department of Pediatric, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology, Wuhan 430030, China.

出版信息

Zhongguo Zhong Yao Za Zhi. 2011 Jul;36(14):1988-92.

PMID:22016974

Abstract

OBJECTIVE

The effect of allitridin on the transcription levels of immediate-early (ie), early(e) and late (1) genes of human cytomegalovirus (HCMV) was investigated in order to explore the mechanism of allitridin against HCMV.

METHOD

Established the models of HCMV AD169 strain infected cells and AD169 strain infected cells treated with allitridin (9.6 mg x L(-1)), and they were compared with the appropriate dose(2.3 mg x L(-1)) of ganciclovir (GCV). All groups of cells were infected at 2.5 multiplicity of infection (MOI), using SYBR Green real-time PCR method to detect the dynamic change of ul122, ul123, ul54 and ul83 mRNA expression at 0.5, 2, 4, 6, 12, 24 h post-infection.

RESULT

The mRNA levels of ul122 and ul123 in AD169 infected cells treated with allitridin at all time points were markedly lower than those of AD169 infected controls (P<0.05), but there were no significant difference of ul122 gene in AD169 infected cells treated with GCV and AD169 infected cells at 0.5-6 h post-infection. The inhibitory rates of allitridin to AD169 ul122 and ul123 mRNA reached 75.2% and 70.4% at 24 h post-infection, respectively. The expression of ul54 mRNA in two drug-treatment groups at all time points were lower than those of AD169 infected cells group (P<0.05). The inhibitory rates of alltridin and GCV to AD169 ul54 mRNA were 45.4% and 27.2% at 24 h post-infection,respectively. The expression of HCMV ul83 mRNA in all groups rapidly increased after 6 h of infection,which is most obvious in AD169 infected cells group. The inhibitory rates of alltridin and GCV to AD169 ul83 mRNA were 45.9% and 26.2% at 24 h post-infection, respectively.

CONCLUSION

Allitridin could effectively suppress the transcription of ie genes (ul122 and ul123) of HCMV AD169 strain, led the expression of mRNA significantly lowerd. It was able to supress the transcription of egene (ul54) and l gene (ul83) too, indicating that HCMV ie genes may be the key target of allitridin against HCMV.

摘要

目的

研究大蒜素对人巨细胞病毒（HCMV）即刻早期（ie）、早期（e）和晚期（l）基因转录水平的影响，以探讨大蒜素抗HCMV的作用机制。

方法

建立HCMV AD169株感染细胞模型以及经大蒜素（9.6mg·L⁻¹）处理的AD169株感染细胞模型，并与适当剂量（2.3mg·L⁻¹）的更昔洛韦（GCV）进行比较。所有细胞组均以2.5感染复数（MOI）进行感染，采用SYBR Green实时荧光定量PCR法检测感染后0.5、2、4、6、12、24h时ul122、ul123、ul54和ul83 mRNA表达的动态变化。

结果

各时间点大蒜素处理的AD169感染细胞中ul122和ul123的mRNA水平均显著低于AD169感染对照组（P<0.05），但感染后0.5 - 6h，GCV处理的AD169感染细胞与AD169感染细胞中ul122基因无显著差异。感染后24h，大蒜素对AD169 ul122和ul123 mRNA的抑制率分别达到75.2%和70.4%。两个药物处理组各时间点ul54 mRNA表达均低于AD169感染细胞组（P<0.05）。感染后24h，大蒜素和GCV对AD169 ul54 mRNA的抑制率分别为45.4%和27.2%。感染6h后，所有组中HCMV ul83 mRNA表达迅速增加，在AD169感染细胞组中最为明显。感染后24h，大蒜素和GCV对AD169 ul83 mRNA的抑制率分别为45.9%和26.2%。