钝顶螺旋藻的细胞浮选采收及生长阶段测定
Harvesting of Spirulina platensis by cellular flotation and growth stage determination.
作者信息
Kim S-G, Choi A, Ahn C-Y, Park C-S, Park Y-H, Oh H-M
机构信息
Biological Resource Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon, Korea.
出版信息
Lett Appl Microbiol. 2005;40(3):190-4. doi: 10.1111/j.1472-765X.2005.01654.x.
AIM
To investigate an effective harvesting method for Spirulina platensis.
METHODS AND RESULTS
Eighty per cent of S. platensis cells in the logarithmic growth phase were harvested by flotation when the cells were set in a static condition for 2 h. The optimum harvesting time was about day 6 of cultivation. The flotation activity of S. platensis cells was enhanced by the addition of NaCl.
CONCLUSIONS
The harvesting of S. platensis by flotation is a cost-effective and straightforward method that can retain the algal quality. The optimum harvesting time of S. platensis can be predicted by the cellular protein to carbon ratio.
SIGNIFICANCE AND IMPACT OF THE STUDY
Flotation harvesting is also applicable to other cyanobacteria with gas vesicles.
目的
研究钝顶螺旋藻的有效采收方法。
方法与结果
当处于对数生长期的钝顶螺旋藻细胞在静态条件下放置2小时后，通过浮选可采收80%的细胞。最佳采收时间约为培养的第6天。添加氯化钠可增强钝顶螺旋藻细胞的浮选活性。
结论
通过浮选采收钝顶螺旋藻是一种经济高效且简便的方法，能够保持藻类品质。钝顶螺旋藻的最佳采收时间可通过细胞蛋白质与碳的比例来预测。
研究的意义与影响
浮选采收也适用于其他具有气泡的蓝细菌。
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