Kim Choong-Jae, Jung Yun-Ho, Oh Hee-Mock
Environmental Biotechnology Research Center, Korea Research Institute of Bioscience and Biotechnology, Daejeon 305-806, Republic of Korea.
J Microbiol. 2007 Apr;45(2):122-7.
Factors indicating culture status of two Spirulina platensis strains were monitored in a batch mode cultivation for 36 days. Changing mode in all factors showed a common turning point, indicating shift of cell or culture status. Mean biomass productivity was highly sustained until day 22, chlorophyll a concentration peaked on day 22, pH value was >12 on day 22, coil number was abruptly shortened on day 22, and floating activity was sustained at greater than 79% after day 22, indicating that day 22 is a criterion reflecting phase-transfer in cell physiology in a batch culture system. Many of these changes may have been caused by increased pH, suggesting that pH control is essential for mass production of S. platensis. Fluctuations in floating activity were likely induced by the number of cellular gas vacuoles. Consequently, coil number per trichome and floating activity of S. platensis could readily act as simple indicators for determination of culture status or harvesting time of cells.
在分批培养模式下，对两种钝顶螺旋藻菌株的培养状态相关因素进行了36天的监测。所有因素的变化模式都显示出一个共同的转折点，表明细胞或培养状态发生了转变。平均生物量生产力在第22天之前一直保持较高水平，叶绿素a浓度在第22天达到峰值，第22天的pH值大于12，藻丝螺旋数在第22天突然缩短，第22天之后漂浮活性持续保持在79%以上，这表明第22天是反映分批培养系统中细胞生理阶段转变的一个标准。这些变化中的许多可能是由pH值升高引起的，这表明pH值控制对于钝顶螺旋藻的大规模生产至关重要。漂浮活性的波动可能是由细胞中气泡的数量引起的。因此，每束藻丝的螺旋数和钝顶螺旋藻的漂浮活性可以很容易地作为确定培养状态或细胞收获时间的简单指标。
