Unsicker K, Reichert-Preibsch H, Wewetzer K
Department of Anatomy and Cell Biology, University of Marburg, F.R.G.
Brain Res Dev Brain Res. 1992 Feb 21;65(2):285-8. doi: 10.1016/0165-3806(92)90191-x.
The multifunctional proteins, basic fibroblast growth factor (bFGF) and ciliary neurotrophic factor (CNTF), share a capacity to promote in vitro and in vivo survival of several, partly overlapping neuron populations. Whether they can affect neurons directly or whether their supportive effects are mediated by non-neuronal cells and their growth factor products has been addressed in this study by establishing single neuron cultures from embryonic chick ciliary ganglia. Cultures with one or two neurons and without any non-neuronal cells were obtained by limiting dilution of ganglionic cell suspensions on 96-well microtiter plates. In the presence of bFGF about 80% of the wells that contained 1 or 2 neurons at the time of seeding, had this (these) neuron(s) maintained after 1 and 5 days. Absence of bFGF resulted in the death of neurons in over 80% of the wells screened. Identical results were obtained with CNTF. These data demonstrate the effectiveness of bFGF and CNTF at the single neuron level, but do not rule out that the factors may act indirectly on neurons, particularly in complex in vitro and in vivo situations.
多功能蛋白碱性成纤维细胞生长因子（bFGF）和睫状神经营养因子（CNTF）具有促进多种部分重叠的神经元群体在体外和体内存活的能力。本研究通过从胚胎鸡睫状神经节建立单神经元培养物，探讨了它们是能直接影响神经元，还是其支持作用由非神经元细胞及其生长因子产物介导。通过在96孔微量滴定板上对神经节细胞悬液进行有限稀释，获得了含有一两个神经元且无任何非神经元细胞的培养物。在存在bFGF的情况下，接种时含有1个或2个神经元的孔中，约80%在1天和5天后仍保留这些神经元。缺乏bFGF导致超过80%被筛选孔中的神经元死亡。CNTF也得到了相同的结果。这些数据证明了bFGF和CNTF在单神经元水平上的有效性，但不排除这些因子可能间接作用于神经元，特别是在复杂的体外和体内情况下。
