Tuttle J B, Mackey T, Steers W D
Department of Urology, University of Virginia Health Sciences Center, Charlottesville 22908.
Neurosci Lett. 1994 May 23;173(1-2):94-8. doi: 10.1016/0304-3940(94)90157-0.
The responsiveness of cultured major pelvic ganglion (MPG) neurons, isolated from adult rats, to nerve growth factor (NGF), basic fibroblastic growth factor (bFGF) and ciliary neuronotrophic factor (CNTF) was tested using in vitro survival assay. MPG neurons respond to NGF with increased survival (+35 +/- 13.3%, mean +/- S.E.), a response completely blocked by antibodies specific to NGF. bFGF (+85 +/- 9.6%) and CNTF (+10.5 +/- 0.5%) also augment survival of MPG neurons in vitro. The effect of bFGF was partially blocked by bFGF antibody. Anti-NGF antibody reduced neuronal survival by 25 +/- 4.1% in conditioned medium from cultures of bladder smooth muscle, suggesting bladder produces NGF. Combining antibodies against NGF and bFGF reduced survival by 19 +/- 0.5% in medium supplemented with bladder extracts, suggesting the extracts contain neurotrophic activity in addition to NGF. These results support the hypothesis that neurons regulating bladder function respond to NGF and other growth factors. Therefore, previously documented changes in bladder neurotrophic factors following hypertrophy, inflammation and injury may elicit growth or change in the autonomic nervous system.
采用体外存活试验，检测从成年大鼠分离出的培养主盆神经节（MPG）神经元对神经生长因子（NGF）、碱性成纤维细胞生长因子（bFGF）和睫状神经营养因子（CNTF）的反应性。MPG神经元对NGF有反应，其存活率增加（+35±13.3%，平均值±标准误），这种反应被NGF特异性抗体完全阻断。bFGF（+85±9.6%）和CNTF（+10.5±0.5%）在体外也能提高MPG神经元的存活率。bFGF抗体可部分阻断bFGF的作用。抗NGF抗体使膀胱平滑肌培养物条件培养基中的神经元存活率降低25±4.1%，提示膀胱可产生NGF。在添加膀胱提取物的培养基中，联合使用抗NGF和bFGF抗体可使存活率降低19±0.5%，提示提取物除了含有NGF外，还含有神经营养活性。这些结果支持以下假说：调节膀胱功能的神经元对NGF和其他生长因子有反应。因此，先前记录的肥大、炎症和损伤后膀胱神经营养因子的变化可能引发自主神经系统的生长或改变。
