用于荧光检测圆二色性的通用椭球面镜装置的开发：消除偏振伪像。

Development of a universal ellipsoidal mirror device for fluorescence detected circular dichroism: elimination of polarization artifacts.

作者信息

Nehira Tatsuo, Tanaka Katsunori, Takakuwa Takashi, Ohshima Chie, Masago Hisashi, Pescitelli Gennaro, Wada Akio, Berova Nina

机构信息

Faculty of Integrated Arts and Sciences, Hiroshima University, 1-7-1, Kagamiyama, Higashi-hiroshima 739-8521, Japan.

出版信息

Appl Spectrosc. 2005 Jan;59(1):121-5. doi: 10.1366/0003702052940459.

DOI:10.1366/0003702052940459

PMID:15720747

Abstract

We have developed an ellipsoidal mirror fluorescence detected circular dichroism (FDCD) device with enhanced detection sensitivity that eliminates the polarization artifact; this is applicable to samples with strongly polarized fluorescence. The device, JASCO FDCD465, has an ellipsoidal mirror structure with a framework consisting of three mirrors (one elliptical and two plane mirrors) that maximally collects light in the FDCD. All assemblies on the device including the ellipsoidal mirror, cylindrical cell, and photomultiplier tube (PMT) are aligned on the chamber-fitting sample mount as an attachment compatible with a standard CD spectropolarimeter. The new FDCD465 device eliminates the polarization artifact caused by anisotropic distribution of the emitted light. It represents a convenient, reliable, and sensitive FDCD attachment to the JASCO J-800 CD spectrometer series that can be used under both isotropic and photoselected conditions.

摘要

我们开发了一种具有增强检测灵敏度的椭球面镜荧光检测圆二色性（FDCD）装置，该装置消除了偏振伪像；这适用于具有强偏振荧光的样品。该装置JASCO FDCD465具有椭球面镜结构，其框架由三个镜子（一个椭圆镜和两个平面镜）组成，可在FDCD中最大程度地收集光。该装置上的所有组件，包括椭球面镜、圆柱形样品池和光电倍增管（PMT），都作为与标准圆二色光谱偏振仪兼容的附件，安装在腔室适配样品架上。新型FDCD465装置消除了由发射光的各向异性分布引起的偏振伪像。它是一种方便、可靠且灵敏的FDCD附件，可用于JASCO J-800圆二色光谱仪系列，并且可以在各向同性和光选择条件下使用。