使用高分辨率¹H磁共振波谱对大鼠突触体和大脑进行定量分析。

Quantitative analysis of rat synaptosomes and cerebrum using high-resolution 1H magnetic resonance spectroscopy.

作者信息

Petroff O A, Burlina A P, Black J, Prichard J W

机构信息

Department of Neurology, Yale University, New Haven, CT 06510.

出版信息

Clin Chim Acta. 1992 Mar 13;206(1-2):137-46. doi: 10.1016/0009-8981(92)90014-h.

DOI:10.1016/0009-8981(92)90014-h

PMID:1572075

Abstract

This study explored the utility of 1H magnetic resonance spectroscopy to study a standard synaptosomally enriched preparation (P2 pellet) made from rat cerebrum. The preparation contained high concentrations of N-acetylaspartate and gamma-aminobutyric acid and low concentrations of glutamine, indicating that they were in fact rich in neuronal cytosol. Synaptosomes contained half the lactate and glutamine of cerebrum. Alanine, aspartate, glutamate, and succinate had the same concentrations in synaptosomes and cerebrum.

摘要

本研究探讨了1H磁共振波谱在研究由大鼠大脑制成的标准突触体富集制剂（P2沉淀）中的应用。该制剂含有高浓度的N-乙酰天门冬氨酸和γ-氨基丁酸，以及低浓度的谷氨酰胺，表明它们实际上富含神经元胞质溶胶。突触体中乳酸和谷氨酰胺的含量是大脑中的一半。丙氨酸、天冬氨酸、谷氨酸和琥珀酸在突触体和大脑中的浓度相同。