酶联免疫吸附法与间接免疫荧光法检测海地农村地区血清中人嗜T淋巴细胞病毒I型抗体的比较

Comparison of enzyme-linked immunosorbent and indirect immunofluorescence assays for the detection of human T-cell lymphotropic virus type-I antibodies in sera from rural Haiti.

作者信息

Grant W, Bia F J, Chacko T M, Jean-Baptiste M, Griffith B P

机构信息

Virology Reference Laboratory, VA Medical Center, West Haven, CT 06516.

出版信息

Diagn Microbiol Infect Dis. 1992 Feb;15(2):121-4. doi: 10.1016/0732-8893(92)90034-q.

DOI:10.1016/0732-8893(92)90034-q

PMID:1572136

Abstract

Serum samples were obtained from 340 healthy individuals without evidence of neurologic disease living in rural Haiti. Sera were screened for antibodies to human T-cell lymphotropic virus type I (HTLV-I) using two commercially available enzyme immunoassays (EIA) and by an indirect immunofluorescence assay (IFA) using a mixture of uninfected H9 cells and HTLV-I-infected MT-2 cells. Repeatedly positive samples were confirmed by Western blot (WB). Results with the two EIA systems were concordant and detected 13 positive samples, each of which was confirmed by WB. Only 9 (69%) of 13 WB-positive sera were detected by IFA, and four additional samples, positive by IFA, could not be confirmed by WB. The prevalence of HTLV-I seropositivity in this selected rural Haitian population was 3.8% (13 of 340).

摘要

从海地农村340名无神经疾病证据的健康个体中采集血清样本。使用两种市售酶免疫测定法（EIA）以及通过使用未感染的H9细胞和HTLV-I感染的MT-2细胞混合物的间接免疫荧光测定法（IFA）对血清进行人类T细胞嗜淋巴细胞病毒I型（HTLV-I）抗体筛查。多次呈阳性的样本通过蛋白质印迹法（WB）进行确认。两种EIA系统的结果一致，检测到13个阳性样本，每个样本均通过WB确认。13个WB阳性血清中只有9个（69%）通过IFA检测到，另外4个IFA阳性样本无法通过WB确认。在这个选定的海地农村人群中，HTLV-I血清阳性率为3.8%（340人中的13人）。