Rangel P L, Lassala A, Gutierrez C G
Departamento de Reproducción, Facultad de Medicina Veterinaria, Universidad Nacional Autónoma de México, Av. Universidad 3000, México DF 04510, Mexico.
Anim Reprod Sci. 2005 Mar;86(1-2):143-51. doi: 10.1016/j.anireprosci.2004.07.002.
The role of testosterone in the ovulatory process in hens has been largely neglected. The aim of the present study was to evaluate if testosterone plays an important role on the ovulatory process in laying hens. The effect of active and passive immunization against testosterone on ovarian follicular development and oviposition was studied. Egg laying percentage was evaluated in hens actively immunized against testosterone-BSA (T-AI; n = 6) or BSA (BSA-AI; n = 6). Oviposition was reduced as antibody titer increased in T-AI hens (r = -0.67; P < 0.01). Ovarian structures were assessed in three animals from each group. Follicles reached preovulatory size in both groups, nonetheless, in T-AI hens follicles at different stages of regression indicated that ovulation was blocked by treatment. In the remaining animals, preovulatory concentrations of progesterone and testosterone were determined. A preovulatory surge release of progesterone, preceded by a testosterone peak, was observed in the BSA-AI group (P < 0.05). In contrast, progesterone in T-AI animals remained at basal concentrations. Whereas, testosterone concentrations were significantly greater in T-AI as compared with BSA-AI animals (P < 0.05). Finally, to study the effect of passive immunization on oviposition, hens were passively immunized (PI) on four occasions, on alternate days with anti-T serum (T-PI; n = 10) or anti-BSA serum (BSA-PI; n = 8). During the 13-day period that preceded treatment, oviposition averaged 94.1%. Forty-eight hours after the first immunization, no egg was laid by 8 out of the 10 T-PI hens. During the 10 days following the first passive immunization, there was a reduction in the laying percentage that was significantly greater in T-PI hens (reduction of 52% in T-PI versus 29% in P-BSA, P < 0.01). In summary, these studies show that testosterone immunization hampers egg-laying without affecting ovarian follicular development, suggesting that testosterone has an important role in the ovulatory process in laying hens.
睾酮在母鸡排卵过程中的作用在很大程度上被忽视了。本研究的目的是评估睾酮是否在蛋鸡排卵过程中发挥重要作用。研究了主动和被动免疫睾酮对卵巢卵泡发育和产卵的影响。对主动免疫睾酮 - 牛血清白蛋白（T - AI；n = 6）或牛血清白蛋白（BSA - AI；n = 6）的母鸡的产蛋率进行了评估。在T - AI母鸡中，随着抗体滴度增加，产卵量减少（r = -0.67；P < 0.01）。对每组三只动物的卵巢结构进行了评估。两组卵泡均达到排卵前大小，然而，在T - AI母鸡中，不同退化阶段的卵泡表明排卵被处理阻断。在其余动物中，测定了排卵前孕酮和睾酮的浓度。在BSA - AI组中观察到孕酮的排卵前激增释放，之前有一个睾酮峰值（P < 0.05）。相比之下，T - AI动物中的孕酮保持在基础浓度。而与BSA - AI动物相比，T - AI动物中的睾酮浓度显著更高（P < 0.05）。最后，为了研究被动免疫对产卵的影响，母鸡每隔一天用抗T血清（T - PI；n = 10）或抗BSA血清（BSA - PI；n = 8）进行四次被动免疫（PI）。在治疗前的13天期间，平均产卵率为94.1%。首次免疫48小时后，10只T - PI母鸡中有8只未产蛋。在首次被动免疫后的10天内，产蛋率下降，T - PI母鸡下降幅度显著更大（T - PI下降52%，而P - BSA下降29%，P < 0.01）。总之，这些研究表明，免疫睾酮会阻碍产蛋但不影响卵巢卵泡发育，这表明睾酮在蛋鸡排卵过程中起重要作用。
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