Department of Animal Science, South China Agricultural University, Guangzhou, China.
Domest Anim Endocrinol. 2011 Aug;41(2):57-66. doi: 10.1016/j.domaniend.2011.03.002. Epub 2011 Apr 28.
The aim of this study was to show a stimulatory role in ovarian follicle development by prolactin (PRL) in chicken hens. In experiment 1, anti-PRL antibodies were generated in hen plasma by intramuscular administrations of recombinant PRL antigen. Egg laying remained at levels lower (P < 0.05) in the PRL-immunized group than in the BSA-immunized group of hens, whereas development of incubation was depressed in the former but not the latter group. Throughout the experiment, plasma PRL concentrations were lower in the PRL-immunized hens than in non-incubating control hens; LH concentrations were similar between the PRL- and BSA-immunized hens until the end of the experiment when LH was lower in the BSA-immunized hens (P < 0.05). In experiment 2, anti-PRL receptor (PRLR) antibodies were raised in hens with the use of immunizations against recombinant PRLR extracellular domain. Immunization against PRLR initially increased the egg-laying rate when measured under the short photoperiod (12 h) but blocked the laying rate increase that occurred in the BSA-immunized control hens when the photoperiod was extended from 12 to 16 h. The development of incubation behavior was not affected by immunization against PRLR nor was plasma PRL or LH concentration. In experiment 3, when the egg-laying rate was depressed in PRL immunization hens, developmental speed of large white follicles was found to be slower than in the BSA-immunized control hens (P < 0.05). These results indicate that immunization against PRL slows down ovarian follicular development and reduces hen egg-laying performance, suggesting that PRL plays a stimulatory role in ovarian follicular development in chicken hens.
本研究旨在展示催乳素（PRL）在母鸡卵巢卵泡发育中的刺激作用。在实验 1 中，通过肌肉内给予重组 PRL 抗原，在母鸡血浆中产生抗 PRL 抗体。与 BSA 免疫组的母鸡相比，PRL 免疫组的母鸡产蛋量较低（P < 0.05），而前者的孵化发育受到抑制，但后者没有。在整个实验过程中，PRL 免疫母鸡的血浆 PRL 浓度低于未孵化对照母鸡；PRL 和 BSA 免疫母鸡的 LH 浓度相似，直到实验结束时，BSA 免疫母鸡的 LH 浓度较低（P < 0.05）。在实验 2 中，使用针对重组 PRLR 细胞外域的免疫接种来产生针对 PRLR 的抗体制剂。针对 PRLR 的免疫接种最初增加了在短光照周期（12 小时）下的产蛋率，但阻止了在光照周期从 12 小时延长到 16 小时时 BSA 免疫对照母鸡产蛋率的增加。针对 PRLR 的免疫接种既不影响孵化行为的发展，也不影响血浆 PRL 或 LH 浓度。在实验 3 中，当 PRL 免疫母鸡的产蛋率下降时，大白卵泡的发育速度比 BSA 免疫对照组的母鸡慢（P < 0.05）。这些结果表明，针对 PRL 的免疫接种会减缓卵巢卵泡的发育并降低母鸡的产蛋性能，表明 PRL 在母鸡的卵巢卵泡发育中发挥刺激作用。
